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Nucleic Acids Research, 1981, Vol. 9, No. 22 5991-6000
© 1981

MOLECULAR BIOLOGY

Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase

Michael Z. Gilman, Janey L. Wiggs and Michael J. Chamberlin

Department of Biochemistry, University of California Berkeley, CA 94720, USA

Received July 24, 1981. RNA polymerase holoenzymes from many bacterial species share a common promoter recognition specificity since they use the same promoter sites on a variety of templates. These promoters generally include sequences homologous to the average sequences -TTGACA- and -TATAATA-, located –35 and –10 base pairs, respectively, upstream of the transcriptional start site. We have isolated a minor form of B. subtilis RNA polymerase in which the normal sigma subunit ({sigma}55) is replaced by a smaller polypeptide ({sigma}28) and which is highly specific for a class of promoter sites not used by the {sigma}55-holoenzyme. Sequencing of two B. subtilis promoter sites used by the {sigma}28-holoenzyme reveals identical sequences at –35 and –10 base pairs from the start site, which are -CTAAA- and -CCGATAT-, respectively. These results confirm that sigma subunit plays a major direct role in promoter sequence recognition, and support a model in which sigma interacts sequentially with –35 and –10 regions, respectively.


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