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Nucleic Acids Research, 1981, Vol. 9, No. 22 6115-6127
© 1981

CHEMISTRY

The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease

Margarete Goppelt, Jörg Langowski, Alfred Pingoud, Wolfgang Haupt, Claus Urbanke, Hubert Mayer* and Günter Maass

Abteilung Biophysikalische Chemie, Zentrum Biochemie, Medizinische Hochschule Hannover D-3000 Hannover 61 *Abteilung Genetik, Gesellschaft für Biotechnologische Forschung D-3300 Braunschweig-Stöckheim, GFR

Received August 25, 1981. The endonucleolytic action of the EcoRI restriction enzyme on the double-stranded oligonucleotide d(GGAATTCC) and the supercoiled plasmid DNA pBR 322 is inhibited by actinomycin D, ethidium bromide, proflavin, distamycin A and netropsin. Half-maximal inhibition is observed at around 100 µM concentrations for the intercalating drugs, and around 0.1 to 1 µM concentrations for netropsin and distamycin A. The inhibitory activity of these drugs can be correlated with their affinity to the oligonucleotide and the plasmid DNA. Since at high concentrations of the drugs a complete inhibition is observed, it is concluded that the effect of the drugs on the stereochemistry of the EcoRI site is such that recognition is excluded.


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S. A. Keatch, T.-J. Su, and D. T. F. Dryden
Alleviation of restriction by DNA condensation and non-specific DNA binding ligands
Nucleic Acids Res., November 1, 2004; 32(19): 5841 - 5850.
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