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Nucleic Acids Research, 1981, Vol. 9, No. 22 6153-6166
© 1981

MOLECULAR BIOLOGY

Antiviral activities of hybrids of two major human leukocyte interferons

Phillip K. Weck*, Sharon Apperson*, Nowell Stebbing*, Patrick W. Gray+, David Leung+, H. Michael Shepard+ and David V. Goeddel+

*Department of Biology Genentech Inc. 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA +Department of Molecular Biology, Genentech Inc. 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA

Received August 3, 1981. Four hybrid human leukocyte interferon (LeIF or IFN-{alpha}) genes have been constructed by in vitro recombination of LeIF-A (IFN-{alpha}2) and LeIF-D (IFN-{alpha}l) genes at common restriction endonuclease sites located within their coding regions. These hybrid genes have been expressed in E. coli under trp promoter control. The interferons produced [LeIF-AD (BgIII]-AD (PvuII),-DA (BgIII),-DA (PvuII)] have antiviral properties distinct from the parental molecules LeIF-A and -D, varying considerably in their abilities to inhibit plaque formation by different viruses in a range of mammalian cells. All six of the cloned LeIFs exhibit the heat stability, pH 2 stability and antigenic specificity of natural leukocyte interferons.


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