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Nucleic Acids Research, 1981, Vol. 9, No. 22 6191-6197
© 1981

ENZYMOLOGY

Is Tb3+ fluorescence enhancement only due to binding to single stranded polynucleotides?

Tomasz Haertléaa,b, Jacek Augustyniaka and Wilhelm Guschlbauerb

aDepartment of Biochemistry, A.Mickiewicz University Fredry 10, PL-61-701 Poznan, Poland bService de Biochimie, Département de Biologie, Centre d'Etudes Nucléaires de Saclay F-91191 Gif-sur-Yvette, France

Received July 14, 1981. Enhancement of Tb3+ fluorescence upon binding to double-stranded ribo- and deoxyribo-duplexes was investigated. It was observed that certain double stranded ribopolynucleotides completely quenched the Tb3+ fluorescence and others did not. It is concluded that the nature of the base in the duplex is critical for this enhancement. Polydeoxyduplexes also showed enhancement of Tb3+ fluorescence, but much higher terbium concentrations were necessary to obtain similar fluorescence signals, indicative of unspecific effects. CD spectra evidenced considerable conformational changes of these duplexes, in particular poly(dG-C).poly(dG-C) which assumes the Z-form in O.1 mM Tb3+.


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