Skip Navigation

This Article
Right arrow Print PDF (623K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Zuidenveg, E.R.P.
Right arrow Articles by Beyreuther, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zuidenveg, E.R.P.
Right arrow Articles by Beyreuther, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 23 6553-6570
© 1981

MOLECULAR BIOLOGY

1H NMR studies of lac-operator DNA fragments

E.R.P. Zuidenveg*, R.M. Scheek*, G. Veeneman+, J.H. van Boom+, R. Kaptein*, H. Rüterjans{dagger} and K. Beyreuther**

*Dep. Physical Chemistry, University of Groningen Groningen +Dep. Organic Chemistry, University of Leiden Leiden, The Netherlands {dagger}Institute of Biophysical Chemistry, University of Frankfurt a. M. Frankfurt a. M. **Institute of Genetics, University of Cologne Cologne, GFR

Received September 29, 1981. The hydrogen-bonded imino protons of a 14 base pair double-stranded DNA fragment comprising one half of the lac operator of E. coli were investigated by 360 MHz 1H NMR. From combined melting studies of this synthetic 14 b.p. fragment and its two constituent 7 b.p. fragments a nearly complete assignment for the low-field proton resonances was obtained. The experimental spectra are compared with calculated spectra and with the spectrum of a 51 b.p. DNA restriction fragment from E. coli containing the complete lac operator. Structural information on these oligonucleotides is presented. This study is a prerequisite for future 1H NMR investigations of the interaction of the lac operator with the lac repressor.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.