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Nucleic Acids Research, 1981, Vol. 9, No. 23 6601-6614
© 1981

MOLECULAR BIOLOGY

The organization of the dihydrofolate reductase gene of baby hamster kidney fibroblasts

Ronald G. Schoner* and John W. Littlefield

Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

Received September 3, 1981.

Using cloned DNA complementary to mouse dihydrofolate reductase (DHFR) mRNA, the organization of the hamster DHFR gene has been determined in two baby hamster kidney (BHK) cell lines, A5 and B1. A5 cells are highly methotrexate-resistant, containing 200-fold more copies of the DHFR gene than do the parental B1 cells. The DHFR gene has the same organization in A5 and B1 cells, suggesting that it has not been altered by the amplification process. The BHK DHFR gene spans a maximum of 10.7 kb and contains at least three introns. Thus the BHK DHFR gene is much smaller than the mouse DHFR gene, which has a minimum size of 42 kb and at least five introns. This striking size difference is probably due to much smaller introns in the BHK DHFR gene.

*Current Address: Department of Biological Sciences, University of Maryland, Catonsville, Maryland, 21228, USA.


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