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Nucleic Acids Research, 1981, Vol. 9, No. 24 6689-6708
© 1981

MOLECULAR BIOLOGY

Molecular cloning and analysis of cDNA sequences derived from polyA+ RNA from barley endosperm: identification of B hordein related clones

B.G. Forde, M. Kreis, M.B. Bahramian, J.A. Matthews, B.J. Miflin, R.D. Thompson, D. Bartels and R.B. Flavell

Biochemistry Department, Rothamsted Experimental Station Harpenden, Herts. AL5 2JQ Cytogenetics Department, Plant Breeding Institute Maris Lane, Trumpington, Cambridge CB2 2LQ, UK

Received November 6, 1981. A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of TT of the B hordein cDNA clones to Northern blots of sizefractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-cl6, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to Bl hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species.


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