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Nucleic Acids Research, 1981, Vol. 9, No. 24 6787-6794
© 1981

CHEMISTRY

Altered mobility of polydeoxyribonucleotides in high resolution polyaciylamide gels due to removal of terminal phosphates

Douglas P. Tapper and David A. Clayton

Department of Pathology, Stanford University School of Medicine Stanford, CA 94305, USA

Received September 28, 1981. We have compared the electrophoretic mobility of a series of polynucleotides differing solely by the presence or absence of a terminal phosphate. As expected, the effect of removal of a single terminal phosphoryl residue on electrophoretic mobility is dependent on the size of the polynucleotide and therefore is not constant. Removal of a phosphoryl residue from polynucleotides shorter than 30 nucleotides reduces the mobility the equivalent of one nucleotide. Between 30 and 50 nucleotides the reduction in mobility is approximately one-half a nucleotide, while above 50 nucleotides in size the effect of phosphate removal approaches zero.


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