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Nucleic Acids Research, 1981, Vol. 9, No. 3 489-501
© 1981

MOLECULAR BIOLOGY

The nudeotide sequence of the Very Low Density Lipoprotein II mRNA from chicken

Bé Wieringa1, Geert AB and Max Gruber

Biochemisch Laboratorium, The University Nijenborgh 16, 9747 AG Groningen, The Netherlands

Received December 15, 1980.

The nucleotide sequence of an almost complete, doublestranded cDNA of chicken Very Low Density Lipoprotein II mRNA, carried in recombinant plasmid pVLDLII 3.33 (Wieringa et al., 1979, 7: 2147–2163) is presented. A stretch of 318 nucleotides codes for the pre-VLDLII polypeptide, which consists of a 24 amino acids signal and a 82 amino acids secreted protein. The coding stretch is flanked by 57 nucleotides in the 5'-leader sequence of the mRNA, and 258 nucleotides in the 3'-non-coding region. Hypothetical selfcomplementary structures of parts of the mRNA are presented.

1Falconberg Court, London W1V 5FG, U.K.


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