Skip Navigation

This Article
Right arrow Print PDF (3511K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Langeveld, S.A.
Right arrow Articles by Weisbeek, P.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Langeveld, S.A.
Right arrow Articles by Weisbeek, P.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 3 545-562
© 1981

ENZYMOLOGY

The nuclease specificity of the bacteriophage øX174 A* protein

S.A. Langeveld1,2, A.D.M. van Mansfeld1,3, A. van der Ende1, J.H. van de Pol4, G.A. van Arkel2 and P.J. Weisbeek2

1Institute of Molecular Biology The Netherlands 2Department of Molecular Cell Biology The Netherlands 3Laboratory for Physiological Chemistry The Netherlands 4Academic Computer Centre Utrecht, State University of Utrecht The Netherlands

Received November 24, 1980. The A* protein of bacteriophage øX174 is a single-stranded DNA specific nuclease. It can cleave øX viral ss DNA in many different places. The position of these sites have been determined within the known øX174 nucleotide sequence (1). From the sequences at these sites it is clear that the A protein recognizes and cleaves at sites that show only partial homology with the origin of RF DNA replication in the øX DNA. Different parts of the origin sequence can be deduced that function as a signal for recognition and cleavage by the A* protein. We conclude that different parts within the DNA recognition domain of the A* protein are functional in the recognition of the origin sequence in single-stranded DNA. The existence of different DNA recognition domains in the A* protein, and therefore also in the A protein, leads to a model that can explain how the A protein performs its multiple function in the øX174 DNA replication process (2).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.