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Nucleic Acids Research, 1981, Vol. 9, No. 3 683-696
© 1981


MOLECULAR BIOLOGY

Nudeotide sequence of mouse satellite DNA

Wolfram Hörz and Werner Altenburger1

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München Goethestrasse 33, 8000 München 2, GFR

Received November 24, 1980.

The nudeotide sequence of uncloned mouse satellite DNA has been determined by analyzing Sau96l restriction fragments that correspond to the repeat unit of the satellite DMA. An unambiguous sequence of 234 bp has been obtained. The sequence of the first 250 bases from dimeric satellite fragments present in Sau96l limit digests corresponds almost exactly to two tandemly arranged monomer sequences including a complete Sau96l site inthe center. This is in agreement with the hypothesis that a low level of divergence which cannot be detected in sequence analyses of uncloned DNA is responsible for the appearance of dimeric fragments. Most of the sequence of the 5% fraction of Sau96 monomers that are susceptible to TaqI has also been determined and has been found to agree completely with the prototype sequence. The monomer sequence is internally repetitious being composed of eight diverged subrepeats. The divergence pattern has interesting implications for theories on the evolution of mouse satellite DNA.


1Falconberg Court. London W1V 5FG, U.K.


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