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Nucleic Acids Research, 1981, Vol. 9, No. 4 895-908
© 1981

MOLECULAR BIOLOGY

Binding of microtubule protein to DNA and chromatin: possibility of simultaneous linkage of microtubule to nucleic acid and assembly of the microtubule structure

Alfredo Villasante, Victor G. Corces*, Rafael Manso-Martínez and Jesús Avila

Centro de Biología Molecular (C.S.I.C. - U.A.M.), Universidad Autónoma de Madrid, Facultad de Ciencias Canto Blanco, Madrid-34, Spain

Received November 17, 1980.

Microtubule protein binds to DNA through microtubule associated polypeptides (MAPs). Among MAPs there is one high molecular weight polypeptide (MAP2) which interacts with DNA fundamentally through certain polynucleotide sequences. This interaction is not affected by the presence of histones and other chromosomal proteins. DNA can associate to assembled microtubules and when a determinate DNA/protein ratio is reached the nucleic acid behaves as a microtubule associated molecule. The nucleic acid fragments which preferentially bind to microtubules have been isolated and characterized. These fragments contain DNA regions enriched in repetitive sequences that hybridize preEerentially to the pericentromeric zone of metaphase chromosomes. These results give further support to the model of interaction microtubule-chromosome based upon the mediator function of the microtubule associated proteins.

*Present address: The Biological Laboratories, Harvard University, Cambridge, MA 02138, USA


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