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Nucleic Acids Research, 1981, Vol. 9, No. 4 921-934
© 1981

MOLECULAR BIOLOGY

Characterization of the yeast tRNA2Ser gene family: genomic organization and DNA sequence

Guy S. Page and Benjamin D. Hall

Department of Biochemistry and Biophysics, University of California at San Francisco San Francisco, CA 94143 Department of Genetics, University of Washington Seattle, WA 98195, USA

Received October 23, 1980. Purified, isolated yeast Formula was used as a hybridization probe to estimate the number of Formula genes in the yeast genome.

Molecular clones of several of the genes were obtained. Three examples were studied in detail with respect to their genomic organization, and DNA sequences were determined for them.

There appear to be eleven Formula genes in the yeast genome. They are neither tandemly repeated nor clustered with other tRNA genes. They contain no intervening sequences.


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