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Nucleic Acids Research, 1981, Vol. 9, No. 4 935-946
© 1981

MOLECULAR BIOLOGY

Use of a cloned double stranded cDNA coding for a major androgen dependent protein in rat seminal vesicle secretion: the effect of testosterone in gene expression

Per-Erik Mansson*, Akio Sugino** and Stephen E. Harris*

*Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences P.O. Box 12233, Research Triangle Park, NC 27709, USA **Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences P.O. Box 12233, Research Triangle Park, NC 27709, USA

Received October 20, 1980. The abundant class of poly(A$)RNA [poly(A$)RNAllS] from rat seminal vesicle was used to synthesize ds-cDNAllS. The ds-cDNAllS was inserted and cloned into the Pst I site of pBR-322 using E. coil RR1 host. Colony filter hybridization and restriction mapping was used to demonstrate that a 620 NTP long insert in a plasmid clone (pSV2) represents the almost full length structural gene coding for a precursor to the seminal vesicle secretion protein IV (SVS IV). The entire insert was sequenced and the coding region was matched with the known amino acid sequence. Most of the signal peptide sequence was derived from the DNA sequence. The insert in pSV2 was labelled and used to study the effect of testosterone on the accumulation of mRNA SVS IV. Administration of testosterone to castrated rats resulted in the induction of mRNA SVS IV from a few molecules per cell to levels of over 100,000 after 96 h of hormone treatment.


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