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Nucleic Acids Research, 1981, Vol. 9, No. 4 993-1004
© 1981

MOLECULAR BIOLOGY

Topography of the E. coli 5S RNA- protein complex as determined by crosslinking with dimethyl suberimidate and dimethyl-3, 3'-dithiobispropionimidate

Thomas G. Fanning* and Robert R. Traut

Department of Biological Chemistry, School of Medicine, University of California Davis, CA 95616, USA

Received December 18, 1980.

5S RNA-protein complexes were prepared in vitro using partially purified E. coli 5S RNA and total E. coli 70S ribosomal proteins. The complexes were isolated from sucrose gradients and shown to contain proteins L5, L18, L25 and a fourth protein not heretofore characterized and designated L31'. The complexes were treated with the crosslinking reagents dimethyl suberimidate and dimethyl-3,3 -dithiobispropionimidate. Both reagents gave identical patterns of crosslinked proteins when analyzed by one-dimensional polyacrylamide/dodecyl-sulfate gel electrophoresis. Dimers of L5-L31', L5-L18 and L18-L18 and a trimer containing L5, L18 and L31' were identified by diagonal polyacrylamide/ dodecylsulfate gel electrophoresis of the proteins crosslinked with dimethyl-3,3'-dithiobispropionimidate. No crosslinking was detected between L25 and the other three proteins.

*Present address of Thomas G. Fanning: Department of Pathology, School of Medicine, University of California, Davis, CA 95616.


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