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Nucleic Acids Research, 1981, Vol. 9, No. 5 1111-1121
© 1981

MOLECULAR BIOLOGY

The nucleotide sequence at the 3'-end of Neurospora crassa 25S-rRNA and the location of a 5.8S-rRNA binding site

John M. Kelly and Robert A. Cox

National Institute for Medical Research The Ridgeway, Mill Hill, London NW7 1AA, UK

Received December 18, 1980. The sequence of 110 nucleotides adjacent to the 3'-end of Neurospora crassa 25S-rRNA has been derived by chemical sequencing methods. Sequences present between 40 and 85 nucleotides of the 3'-end were found to complement sequences at the 3'-and 5'-ends of 5.8S-rRNA. Interaction was shown to occur between 5.85-rRNA and a specific 3'-terminal fragment of 85 nucleotides derived from 25s-rRNA. We have also demonstrated that the nucleotide sequence at the 3'-end of N. crassa 5.8S-rRNA (-UCAUUOH) is different from the published sequence (-UUUUOH) which was derived from rDNA.


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