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Nucleic Acids Research, 1981, Vol. 9, No. 5 1241-1250
© 1981

CHEMISTRY

Conformational changes of poly(dG-dC). poly(dG-dC) modified by the carcinogen N-acetoxy-N-acetyl-2-aminofluorene

Evelyne Sage and Marc Leng

Centre de Biophysique Moléculaire, C.N.R.S., 1 A, avenue de la Recherche Scientifique 45045 Orleans Cédex, France

Received December 16, 1980. Poly(dG-dC).poly(dG-dC) was modified by the reaction with N-acetoxy-N- acetyl-2-aminofluorene. The conformations of poly(dG-dC).poly(dG-dC) and of poly (G-C)AAF were studied by circular dichroism under various experimental conditions. In 95 % ethanol, the two polynucleotides adopt the A-form. In 3.9 M LiCl, the transition B-form-C-form is observed with poly(dG-dC).poly(dG-dC) but not with poly d(G-C)AAF. In 1 mM phosphate buffer, poly d(G-C)AAF behaves as a mixture of B- and Z-form, the relative percentages depending upon the amounts of modified bases. The percentage of Z-form is decreased by addition of EDTA and is increased by addition of Mg++. Spermine favors the Z-form in modified and unmodified polynucleotides. No defect in the double helix of poly d(G-C)AAF is detected by SI endonuclease.


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