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Nucleic Acids Research, 1981, Vol. 9, No. 6 1323-1338
© 1981

MOLECULAR BIOLOGY

Structural analysis of the three vitellogenin genes in Drosophila melanogaster

D.Christie Riddell, Michael J. Higgins, B.Jane McMillan and Bradley N. White

Group in Eukaryotic Molecular Biology and Evolution, Department of Biology, Queen's University Kingston, Ontario, Canada

Received January 5, 1981. Genomic fragments coding for sequences expressed as abundant mRNA in female Drosophila melanogaster were isolated from a lambda library. Hybridization of these clones to polytene chromosomes, in situ, identified four which mapped to X chromosomal region 9A to 9B, the locus for yolk proteins 1 and 2 (Yp1,2) and two which mapped to 12A6–7 to 12D3, the locus for Yp3. These clones were mapped with restriction enzymes, and the coding regions and regions of homology determined by Southern blots probed with cDNA, 5'-end-labelled RNA and nick-translated DNA. Heteroduplex and R-loop mapping confirmed that three of the clones carried two genes separated by about 1.4 kb and oriented in opposite directions. Southern blots probed with cDNA made from alkali-hydrolyzed RNA showed that these genes had their 5' ends next to each other. All 3 genes show homology to each other and have a main coding region of about 1.35 kb, the approximate size for the mRNAs.


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