Skip Navigation

This Article
Right arrow Print PDF (999K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hamming, J.
Right arrow Articles by Gruber, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamming, J.
Right arrow Articles by Gruber, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 6 1339-1350
© 1981

MOLECULAR BIOLOGY

Electron microscopic analysis of transcription of a ribosomal RNA operon of E. coli

Jantien Hamming, Annika Arnberg, Geert AB and Max Gruber

Biochemisch Laboratorium, The University Nijenborgh 16, 9747 AG Groningen, The Netherlands

Received January 2, 1981. Transcription in vitro of the E. coli ribosomal RNA operon, rrnE, was analysed by electron microscopy. The transcription initiation sites of the two rrnE promoters in tandem, P1 and P2, were mapped and the transcription from both sites was compared. The first and the second transcription initiation site are about equally used when all nucleotides are present at 200 µM. Lowering the concentration of the second promoter's start nucleotide CTP to 3 µM reduces the use of the P2 site sharply. At all CTP concentrations used the nascent RNA chains from P1 are in the average longer than those from P2 after a fixed transcription time. Most probably, this difference is caused by a longer average interval before formation of the productive complex with the second promoter.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
V. Epshtein and E. Nudler
Cooperation Between RNA Polymerase Molecules in Transcription Elongation
Science, May 2, 2003; 300(5620): 801 - 805.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.