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Nucleic Acids Research, 1981, Vol. 9, No. 7 1599-1614
© 1981

MOLECULAR BIOLOGY

An autoradiographic demonstration of nuclear DNA replication by DNA polymerase {alpha} and of mitochondrial DNA synthesis by DNA polymerase {gamma}

M. Geuskens1,2, N. Hardt1, G. Pedrali-Noy3 and S. Spadari3

1Department of Molecular Biology, Université Libre de Bruxelles 67 Rue des Chevaux, 1640 Rhode-St-Genèse, Belgium 2Institut de Recherches Scientifiques sur le Cancer 94800 Villejuif, France 3Laboratorio de Genetica Biochimica ed Evoluzionistica CNR, 27100 Pavia, Italy

Received January 29, 1981. The incorporation of thymidine into the DNA of eukaryotic cells is markedly depressed, but not completely inhibited, by aphidicolin, a highly specific inhibitor of DNA polymerase {alpha}. An electron microscope autoradiographic analysis of the synthesis of nuclear and mitochondrial DNA in vivo in Concanavalin A stimulated rabbit spleen lymphocytes and in Hamster cell cultures , in the absence and in the presence of aphidicolin, revealed that aphidicolin inhibits the nuclear but not the mitochondrial DNA replication. We therefore conclude that DNA polymerase {alpha} performs the synchronous bidirectional replication of nuclear DNA and that DNA polymerase {gamma}, the only DNA polymerase present in the mitochondria, performs the "strand displacement" DNA synthesis of these organelles.


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