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Nucleic Acids Research, 1981, Vol. 9, No. 8 1853-1872
© 1981

MOLECULAR BIOLOGY

Isolation and characterization of cloned human DNA fragments carrying reiterated sequences common to both autosomes and the X chromosome

Barbara J. Schmeckpeper*, Huntington F. Willard and Kirby D. Smith*

Traylor Building, Room 933 Division of Medical Genetics, Department of Medicine, The Johns Hopkins University School of Medicine Baltimore, MD 21205, USA *The Howard Hughes Medical Institute Baltimore, MD 21205, USA

Received January 15, 1981. Several recombinants were identified and purified from a cloned library of human DNA by virtue of their homology to DNA from a mouse-human hybrid cell line containing a single human chromosome, the X, and their lack of homology to mouse DNA. Three recombinants were characterized in detail, and all were homologous to reiterated DNA from the human X chromosome. These recombinants also were homologous to reiterated sequences on one or more human autosomes and, therefore, were not X chromosome specific. The recombinant DNA fragments homologous to human reiterated X DNA were the same fragments homologous to human reiterated autosomal DNA. Digestion of genomic DNAs with several restriction enzymes revealed that the pattern of fragments homologous to one recombinant, {lambda}Hb2, was the same on autosomes as on the X chromosome, suggesting that the molecular organization of these elements on the X is not distinct from their organization on autosomes.


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