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Nucleic Acids Research, 1981, Vol. 9, No. 9 2187-2194
© 1981


MOLECULAR BIOLOGY

The atp operon: nucleotide sequence of the region encoding the {alpha}-subunit of Escherichia coli ATP-synthase

N.J. Gay and J.E. Walker

The Laboratory of Molecular Biology, The MRC Centre Hills Road, Cambridge CB2 2QH, UK

Received March 6, 1981. Part of the atp (or unc) operon encoding the {alpha}, ß, {gamma}, {delta} and {varepsilon} subunits of Escherichia coli ATP-synthase has been cloned into the plasmid pACYC184. The DNA coding for the largest of these proteins, the a subunit, has been sequenced by cloning into the bacteriophage M13 and sequencing with dideoxy nucleotide chain terminators. It comprises 1539 nucleotides corresponding to a protein of 513 amino acids.


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