Nucleic Acids Research Advance Access first published online on September 20, 2006
This version published online on October 6, 2006
Nucleic Acids Research, doi:10.1093/nar/gkl671
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© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Genomics |
Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform
Genomics-Proteomics-Systemsbiology, Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik Nobelstraße 12, 70569 Stuttgart, Germany 1 Functional Genome Analysis Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580 69120 Heidelberg, Germany 2 Applied Biosystems Foster City, USA
*To whom correspondence should be addressed. Tel: +49 711 9704044; Fax: +49 711 9704200; Email: nicole.hauser{at}igb.fraunhofer.de
Received July 26, 2006. Revised August 29, 2006. Accepted September 1, 2006.
L-DNA is the perfect mirror-image form of the naturally occurring D-conformation of DNA. Therefore, L-DNA duplexes have the same physical characteristics in terms of solubility, duplex stability and selectivity as D-DNA but form a left-helical double-helix. Because of its chiral difference, L-DNA does not bind to its naturally occurring D-DNA counterpart, however. We analysed some of the properties that are typical for L-DNA. For all the differences, L-DNA is chemically compatible with the D-form of DNA, so that chimeric molecules can be synthesized. We take advantage of the characteristics of L-DNA toward the establishment of a universal microarray that permits the analysis of different kinds of molecular diagnostic information in a single experiment on a single platform, in various combinations. Typical results for the measurement of transcript level variations, genotypic differences and DNA–protein interactions are presented. However, on the basis of the characteristic features of L-DNA, also other applications of this molecule type are discussed.
The authors wish to be known that, in their opinion, the first two authors should be regarded as joint First Authors
The history dates have been corrected.
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