Skip Navigation



Nucleic Acids Research Advance Access published online on August 17, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm578
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (5331K) Freely available
Right arrow Screen PDF (768K) Freely available
Right arrowOA All Versions of this Article:
35/16/5581    most recent
gkm578v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Léger, M.
Right arrow Articles by Brakier-Gingras, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Léger, M.
Right arrow Articles by Brakier-Gingras, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

The three transfer RNAs occupying the A, P and E sites on the ribosome are involved in viral programmed -1 ribosomal frameshift

Mélissa Léger, Dominic Dulude, Sergey V. Steinberg and Léa Brakier-Gingras*

Département de Biochimie, Université de Montréal, Montréal, Québec, Canada, H3T 1J4

*To whom correspondence should be addressed. Tel: 514 343 6316; Fax: 514 343 2210; Email: lea.brakier.gingras{at}umontreal.ca

Received May 30, 2007. Revised June 28, 2007. Accepted July 13, 2007.

The -1 programmed ribosomal frameshifts (PRF), which are used by many viruses, occur at a heptanucleotide slippery sequence and are currently thought to involve the tRNAs interacting with the ribosomal P- and A-site codons. We investigated here whether the tRNA occupying the ribosomal E site that precedes a slippery site influences -1 PRF. Using the human immunodeficiency virus type 1 (HIV-1) frameshift region, we found that mutating the E-site codon altered the -1 PRF efficiency. When the HIV-1 slippery sequence was replaced with other viral slippery sequences, mutating the E-site codon also altered the -1 PRF efficiency. Because HIV-1 -1 PRF can be recapitulated in bacteria, we used a bacterial ribosome system to select, by random mutagenesis, 16S ribosomal RNA (rRNA) mutations that modify the expression of a reporter requiring HIV-1 -1 PRF. Three mutants were isolated, which are located in helices 21 and 22 of 16S rRNA, a region involved in translocation and E-site tRNA binding. We propose a novel model where -1 PRF is triggered by an incomplete translocation and depends not only on the tRNAs interacting with the P- and A-site codons, but also on the tRNA occupying the E site.

Present address: Dominic Dulude, Centre de recherche, Hôpital Ste-Justine, 3175, chemin de la Côte-Ste-Catherine, Montréal, Québec, Canada H3T 1C5


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
D. Dulude, G. Theberge-Julien, L. Brakier-Gingras, and N. Heveker
Selection of peptides interfering with a ribosomal frameshift in the human immunodeficiency virus type 1
RNA, May 1, 2008; 14(5): 981 - 991.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.