Skip Navigation



Nucleic Acids Research Advance Access published online on April 5, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn152
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (9387K) Freely available
Right arrow Screen PDF (513K) Freely available
Right arrowOA All Versions of this Article:
36/9/3031    most recent
gkn152v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lu, Q.
Right arrow Articles by Xiang, H.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, Q.
Right arrow Articles by Xiang, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation

Qiuhe Lu1,2, Jing Han1,2, Ligang Zhou1,2, James A. Coker3, Priya DasSarma3, Shiladitya DasSarma3 and Hua Xiang1,*

1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 2Graduate University of Chinese Academy of Sciences, Beijing, People's Republic of China and 3University of Maryland Biotechnology Institute, Center of Marine Biotechnology, Baltimore, MD 21202, USA

*To whom correspondence should be addressed. Tel: +86 10 6480 7472; Fax: +86 10 6480 7472; Email: xiangh{at}sun.im.ac.cn

Received December 6, 2007. Revised March 18, 2008. Accepted March 18, 2008.

Multiple general transcription factors (GTFs), TBP and TFB, are present in many haloarchaea, and are deemed to accomplish global gene regulation. However, details and the role of GTF-directed transcriptional regulation in stress response are still not clear. Here, we report a comprehensive investigation of the regulatory mechanism of a heat-induced gene (hsp5) from Halobacterium salinarum. We demonstrated by mutation analysis that the sequences 5' and 3' to the core elements (TATA box and BRE) of the hsp5 promoter (Phsp5) did not significantly affect the basal and heat-induced gene expression, as long as the transcription initiation site was not altered. Moreover, the BRE and TATA box of Phsp5 were sufficient to render a nonheat-responsive promoter heat-inducible, in both Haloferax volcanii and Halobacterium sp. NRC-1. DNA–protein interactions revealed that two heat-inducible GTFs, TFB2 from H. volcanii and TFBb from Halobacterium sp. NRC-1, could specifically bind to Phsp5 likely in a temperature-dependent manner. Taken together, the heat-responsiveness of Phsp5 was mainly ascribed to the core promoter elements that were efficiently recognized by specific heat-induced GTFs at elevated temperature, thus providing a new paradigm for GTF-directed gene regulation in the domain of Archaea.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.