Skip Navigation



Nucleic Acids Research Advance Access published online on September 29, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn629
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (547K) Freely available
Right arrow Screen PDF (305K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
36/19/6155    most recent
gkn629v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Okuda, K.
Right arrow Articles by Shikanai, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okuda, K.
Right arrow Articles by Shikanai, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

Amino acid sequence variations in Nicotiana CRR4 orthologs determine the species-specific efficiency of RNA editing in plastids

Kenji Okuda1, Yuya Habata2, Yoshichika Kobayashi2 and Toshiharu Shikanai1,*

1Department of Botany, Graduate School of Science, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan and 2Graduate School of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan

*To whom correspondence should be addressed. Tel: + 81 75 753 4247; Fax: + 81 75 753 4257; Email: shikanai{at}pmg.bot.kyoto-u.ac.jp

Received August 1, 2008. Revised September 10, 2008. Accepted September 12, 2008.

In flowering plants, RNA editing is a posttranscriptional process that converts specific C to U in organelle mRNAs. Nicotiana tabacum is an allotetraploid species derived from the progenitors of Nicotiana sylvestris and Nicotiana tomentosiformis. These Nicotiana species have been used as a model for understanding the mechanism and evolution of RNA editing in plastids. In Nicotiana species, the ndhD-1 site is edited to create the translational initiation codon of ndhD that encodes a subunit of the NAD(P)H dehydrogenease (NDH) complex. An analysis of this RNA editing revealed that editing efficiency in N. tomentosiformis is lower (15%) than that in N. tabacum (42%) and N. sylvestris (37%). However, this level of editing is sufficient for accumulating the NDH complex and its activity. The heterogous complementation of Arabidopsis crr4-3 mutant, in which RNA editing of ndhD-1 is completely impaired, with CRR4 orthologous genes derived from Nicotiana species suggested that the reduction in editing efficiency in N. tomentosiformis is caused by amino acid variations accumulating in CRR4.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol PlantHome page
M. Suorsa, S. Sirpio, and E.-M. Aro
Towards Characterization of the Chloroplast NAD(P)H Dehydrogenase Complex
Mol Plant, July 21, 2009; (2009) ssp052v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Zehrmann, D. Verbitskiy, J. A. van der Merwe, A. Brennicke, and M. Takenaka
A DYW Domain-Containing Pentatricopeptide Repeat Protein Is Required for RNA Editing at Multiple Sites in Mitochondria of Arabidopsis thaliana
PLANT CELL, February 1, 2009; 21(2): 558 - 567.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Okuda, A.-L. Chateigner-Boutin, T. Nakamura, E. Delannoy, M. Sugita, F. Myouga, R. Motohashi, K. Shinozaki, I. Small, and T. Shikanai
Pentatricopeptide Repeat Proteins with the DYW Motif Have Distinct Molecular Functions in RNA Editing and RNA Cleavage in Arabidopsis Chloroplasts
PLANT CELL, January 1, 2009; 21(1): 146 - 156.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.