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Nucleic Acids Research Advance Access published online on December 5, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn975
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© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

Multiplex single-base extension typing to identify nuclear genes required for RNA editing in plant organelles

Mizuki Takenaka* and Axel Brennicke

Molekulare Botanik, Universität Ulm, 89069 Ulm, Germany

*To whom correspondence should be addressed. Tel: + 49 731 502 2616; Fax: + 49 731 502 2626; Email: mizuki.takenaka{at}uni-ulm.de

Received October 15, 2008. Revised November 19, 2008. Accepted November 19, 2008.

We developed a multiplex single-base extension single-nucleotide polymorphism-typing procedure for screening large numbers of plants for mutations in mitochondrial RNA editing. The high sensitivity of the approach detects changes in the RNA editing status generated in total cellular cDNA from pooled RNA preparations of up to 50 green plants. The method has been employed to tag several nuclear encoded genes required for RNA editing at specific sites in mitochondria of Arabidopsis thaliana. This approach will allow large-scale screening for mutations in genes encoding trans-factors for many types of RNA editing as well as for other RNA modifications.


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