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Nucleic Acids Research Advance Access published online on October 20, 2009
Nucleic Acids Research, doi:10.1093/nar/gkp856
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© The Author(s) 2009. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Genomics

Expression profiling of Drosophila mitochondrial genes via deep mRNA sequencing

Tatiana Teixeira Torres1,*, Marlies Dolezal1, Christian Schlötterer1 and Birgit Ottenwälder2

1Institut für Populationsgenetik, Veterinärmedizinische Universität Wien, Vienna, Austria and 2Eurofins Medigenomix GmbH, Martinsried, Germany

*To whom correspondence should be addressed. Tel: +55 19 35211141; Fax: +55 19 35211089; Email: tttorres{at}unicamp.br

Received July 13, 2009. Revised September 24, 2009. Accepted September 24, 2009.

Mitochondria play an essential role in several cellular processes. Nevertheless, very little is known about patterns of gene expression of genes encoded by the mitochondrial DNA (mtDNA). In this study, we used next-generation sequencing (NGS) for transcription profiling of genes encoded in the mitochondrial genome of Drosophila melanogaster and D. pseudoobscura. The analysis of males and females in both species indicated that the expression pattern was conserved between the two species, but differed significantly between both sexes. Interestingly, mRNA levels were not only different among genes encoded by separate transcription units, but also showed significant differences among genes located in the same transcription unit. Hence, mRNA abundance of genes encoded by mtDNA seems to be heavily modulated by post-transcriptional regulation. Finally, we also identified several transcripts with a noncanonical structure, suggesting that processing of mitochondrial transcripts may be more complex than previously assumed.

Present address: Tatiana Teixeira Torres, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, CP 6010, 13083-875 Campinas, SP, Brazil.


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