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Nucleic Acids Research Advance Access published online on October 28, 2009
Nucleic Acids Research, doi:10.1093/nar/gkp869
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© The Author(s) 2009. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

Involvement of the nuclear cap-binding protein complex in alternative splicing in Arabidopsis thaliana

Katarzyna Dorota Raczynska1, Craig G. Simpson2, Adam Ciesiolka1, Lukasz Szewc1, Dominika Lewandowska2, Jim McNicol3, Zofia Szweykowska-Kulinska1, John W. S. Brown2,4,* and Artur Jarmolowski1,*

1Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznan, Poland, 2Genetics Programme, Scottish Crop Research Institute (SCRI), 3BioSS and 4Division of Plant Sciences, University of Dundee at SCRI, Dundee DD2 5DA, Scotland, UK

*To whom correspondence should be addressed. Tel: +48 61 829 5959; Fax: +48 61 829 5949; Email: artjarmo{at}amu.edu.pl

Correspondence may also be addressed to John W. S. Brown. Tel: +44 1382 568533; Fax: +44 1382 562426; Email: j.w.s.brown{at}dundee.ac.uk

Received June 25, 2009. Revised September 11, 2009. Accepted September 29, 2009.

The nuclear cap-binding protein complex (CBC) participates in 5' splice site selection of introns that are proximal to the mRNA cap. However, it is not known whether CBC has a role in alternative splicing. Using an RT–PCR alternative splicing panel, we analysed 435 alternative splicing events in Arabidopsis thaliana genes, encoding mainly transcription factors, splicing factors and stress-related proteins. Splicing profiles were determined in wild type plants, the cbp20 and cbp80(abh1) single mutants and the cbp20/80 double mutant. The alternative splicing events included alternative 5' and 3' splice site selection, exon skipping and intron retention. Significant changes in the ratios of alternative splicing isoforms were found in 101 genes. Of these, 41% were common to all three CBC mutants and 15% were observed only in the double mutant. The cbp80(abh1) and cbp20/80 mutants had many more changes in alternative splicing in common than did cbp20 and cbp20/80 suggesting that CBP80 plays a more significant role in alternative splicing than CBP20, probably being a platform for interactions with other splicing factors. Cap-binding proteins and the CBC are therefore directly involved in alternative splicing of some Arabidopsis genes and in most cases influenced alternative splicing of the first intron, particularly at the 5' splice site.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.


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