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Nucleic Acids Research Advance Access published online on October 21, 2009
Nucleic Acids Research, doi:10.1093/nar/gkp874
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© The Author(s) 2009. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Database Issue

REBASE—a database for DNA restriction and modification: enzymes, genes and genomes

Richard J. Roberts*, Tamas Vincze, Janos Posfai and Dana Macelis

New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938, USA

*To whom correspondence should be addressed. Tel: +1 978 380 7405; Fax: +1 978 380 7406; Email: roberts{at}neb.com

Received September 14, 2009. Revised September 29, 2009. Accepted September 30, 2009.

REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction–modification (R–M). It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal and sequence data. Experimentally characterized homing endonucleases are also included. The fastest growing segment of REBASE contains the putative R–M systems found in the sequence databases. Comprehensive descriptions of the R–M content of all fully sequenced genomes are available including summary schematics. The contents of REBASE may be browsed from the web (http://rebase.neb.com) and selected compilations can be downloaded by ftp (ftp.neb.com). Additionally, monthly updates can be requested via email.


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