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Nucleic Acids Research Advance Access published online on November 11, 2009
Nucleic Acids Research, doi:10.1093/nar/gkp932
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© The Author(s) 2009. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

Specific local induction of DNA strand breaks by infrared multi-photon absorption

D. Träutlein1, M. Deibler2, A. Leitenstorfer1 and E. Ferrando-May2,*

1Department of Physics and 2Department of Biology and Center for Applied Photonics, University of Konstanz, D-78457 Konstanz, Germany

*To whom correspondence should be addressed. Tel: +49 7531 884054; Fax: +49 7531 4005; Email: elisa.may{at}uni-konstanz.de

Received June 26, 2009. Revised October 6, 2009. Accepted October 11, 2009.

Highly confined DNA damage by femtosecond laser irradiation currently arises as a powerful tool to understand DNA repair in live cells as a function of space and time. However, the specificity with respect to damage type is limited. Here, we present an irradiation procedure based on a widely tunable Er/Yb : fiber femtosecond laser source that favors the formation of DNA strand breaks over that of UV photoproducts by more than one order of magnitude. We explain this selectivity with the different power dependence of the reactions generating strand breaks, mainly involving reactive radical intermediates, and the direct photochemical process leading to UV-photoproducts. Thus, localized multi-photon excitation with a wavelength longer than 1 µm allows for the selective production of DNA strand breaks at sub-micrometer spatial resolution in the absence of photosensitizers.


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