©
1996 Oxford University Press
2618-2619
Footnote
Fractionation of nucleic acids into single-stranded and double-stranded forms
Fractionation of nucleic acids into single-stranded and double-stranded forms
Marcel
Beld
,
Cees
Sol
,
Jaap
Goudsmit
and
René
Boom
*
Department of Human Retrovirology, Academic Medical Center, University of
Amsterdam, Meibergdreef 15,
1105 AZ
Amsterdam
,
The Netherlands
Received March 11, 1996;
Revised and Accepted May 15, 1996
We describe a rapid and efficient procedure for the fractionation of mixtures of
nucleic acids (NA) into double-stranded (ds) and single-stranded (ss) forms regardless of the nature of the nucleic acid
(DNA or RNA). The procedure is based on the differential binding of ds- and ss-NA forms to silica particles in different lysis/binding buffers
which have in common that they contain a high concentration of the chaotropic agent guanidinium thiocyanate (GuSCN).
Previously we reported on a procedure (protocol Y) for the routine purification
of total NA from clinical specimens (
1
). The method . . . [Full Text of this Article]
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