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© 1996 Oxford University Press 394-395

Footnote

RNA fingerprinting by molecular indexing

RNA fingerprinting by molecular indexing Kikuya Kato

Okayama Cell Switching Project, ERATO, JRDC, Pasteur Building 4F, 103-5 Tanakamonzencho, Sakyo-ku , Kyoto 606, Japan

Received November 2, 1995 ; Accepted November 27, 1995

Class IIS restriction enzymes, a subgroup of class II, cleave DNA at a precise location outside their recognition sites, and produce overhangs of unknown sequences ( 1 ). Molecular indexing is a series of techniques designed to characterize DNA fragments by these unknown sequences ( 2 - 4 ). I applied this principle for description of the total mRNA population using a 3' end cDNA fragment generated by class IIS restiction enzymes ( 5 ). The method is based on the finding that Escherichia coli DNA ligase discriminates three nucleotides adjacent to the joining site. Fragments are discriminated by a library of 64 adaptors for all possible overhangs, and selected fragments . . . [Full Text of this Article]

ACKNOWLEDGEMENT

REFERENCES


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