Compilation of tRNA sequences and sequences of tRNA genes
Compilation of tRNA sequences and sequences of tRNA genes
Mathias
Sprinzl*
,
Clemens
Steegborn
,
Frank
Hübel
1
and
Sergey
Steinberg
+
Laboratorium für Biochemie, Universität Bayreuth, 95440
Bayreuth
,
Germany
and
1
Institut für Landwirtschaftliche Botanik, Universität Bonn, Meckenheimer Allee 176, 53115
Bonn
,
Germany
Received September 5, 1995
;
Accepted September 19, 1995
INTRODUCTION
The 1995 compilation contains 2700 sequences of tRNAs and tRNA genes. The last
edition which appeared two years ago (
1
) was supplemented by 694 new sequences. The sequences of tRNA mutants and of
tRNAs originating from transformed or differentiated cells were not considered.
The tRNAs included in the compilation are listed in Table
1
. Each tRNA or tRNA gene is specified by the (abbreviated) name of the organism
from which it was isolated and a four digit code: the first three digits
identify the organism, the last digit specifies the particular isoacceptor. The
amino acid specificity of the tRNA is indicated by a one-letter amino acid code. The tRNAs coding for selenocysteine were annotated
with the letter Z. Initiator tRNAs are annotated with the letter X.
Table 1
.
List of tRNA sequences and sequences of tRNA genes included in the compilation
The sequences, references and footnotes of tRNAs and tRNA genes listed in Table
1
are deposited in the European Bioinformatics Institute (EBI) Data Library. The
references are restricted to the first complete publication of the sequence
unless additional information (e.g. base modification, corrections, etc.) was
later obtained. In such cases additional references were added.
In order to facilitate a computer analysis an alignment is used which is most
compatible with the tRNA phylogeny and known three-dimensional structures of tRNA. The corresponding numbering system is
shown in Figure
1
.
ACKNOWLEDGEMENTS
This project was supported by Fonds der Chemischen Industrie, Deutsche
Forschungsgemeinschaft, Project Sp 243/5-1, and Molekulargenetischer Arbeitskreis Rhein/Main e.V.
REFERENCES
1 Steinberg S., Misch A. and Sprinzl, M. (1993) Nucleic Acids Res.21, 3011-3015.
2 Limbach P.A., Crain P.F. and McCloskey, J.A. (1994) Nucleic Acids Res. 22, 2183-2196.
3 Steinberg S.V. and Kisselev L.L. (1992) Biochimie74, 337-351.
*
To whom correspondence should be addressed
+
Present address: Université de Montréal, Faculté de Médicine, Départment de Biochimie, C.P. 6128,
Succursale Centre-Ville, Montréal, Québec H3C 3J7, Canada (on leave from Engelhardt Institute of
Molecular Biology, Vavilova 32, Moscow 117984, Russia)
