Nucleic Acids Research, 2001, Vol. 29, No. 1 137-140
© 2001 Oxford University Press
RefSeq and LocusLink: NCBI gene-centered resources
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 38A Room 6N605, 8600 Rockville Pike, Bethesda, MD 20894 USA
Received October 2, 2000; Accepted October 4, 2000.
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ABSTRACT |
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 TOP ABSTRACT BACKGROUNDEXPANDED AND NEW FEATURESGROWTH AND MAINTENANCEREFERENCES |
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Thousands of genes have been painstakingly identified and characterized a few genes at a time. Many thousands more are being predicted by large scale cDNA and genomic sequencing projects, with levels of evidence ranging from supporting mRNA sequence and comparative genomics to computing ab initio models. This, coupled with the burgeoning scientific literature, makes it critical to have a comprehensive directory for genes and reference sequences for key genomes. The NCBI provides two resources, LocusLink and RefSeq, to meet these needs. LocusLink organizes information around genes to generate a central hub for accessing gene-specific information for fruit fly, human, mouse, rat and zebrafish. RefSeq provides reference sequence standards for genomes, transcripts and proteins; human, mouse and rat mRNA RefSeqs, and their corresponding proteins, are discussed here. Together, RefSeq and LocusLink provide a non-redundant view of genes and other loci to support research on genes and gene families, variation, gene expression and genome annotation. Additional information about LocusLink and RefSeq is available at http://www.ncbi.nlm.nih.gov/LocusLink/.
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BACKGROUND |
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TOPABSTRACTBACKGROUNDEXPANDED AND NEW FEATURESGROWTH AND MAINTENANCEREFERENCES |
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LocusLink maintains descriptive information about loci including nomenclature, database identifiers (ID), disease associations, map positions and sequence accessions. These associations are then used to compute connections to other NCBI resources, to both facilitate navigation and enhance opportunities for new discoveries. LocusLink data are continuously augmented and reviewed through the combined efforts of NCBI staff and successful collaborations with the Human Gene Nomenclature Committee (HGNC) (1), Online Mendelian Inheritance in Man (OMIM) (2), Mouse Genome Database (MGD) (3), Rat Genome Database (RGD), FlyBase (4) and Zebrafish (ZFIN) (5) groups. This compilation is freely distributed via the LocusLink web site and by FTP. LocusLink queries can be based on text terms (such as a protein or disease name), gene symbols, sequence accessions and database IDs (e.g., MIM or EC numbers). More complex operations such as wild cards (e.g., ABC*), field restriction (e.g., 123456[mim]) and booleans (e.g., ABC* AND 17[chr]) are also supported.
The NCBI reference sequences (RefSeq) provide standards for complete genomic nucleic acids, assembled contigs, transcripts and proteins (see Table 1). RefSeq records are derived from GenBank and the literature to provide a non-redundant set of sequences that facilitate sequence identification and information retrieval (6). Human, mouse and rat RefSeq mRNAs, and their corresponding proteins, are the focus of this discussion; these sequences can be accessed through the NCBI Entrez retrieval system (7), BLAST (8), FTP and LocusLink.
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TOPABSTRACTBACKGROUNDEXPANDED AND NEW FEATURESGROWTH AND MAINTENANCEREFERENCES |
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LocusLink
The locus-to-sequence association maintained in LocusLink is used to provide reciprocal connections among LocusLink, RefSeq and several content-rich resources at NCBI including the Conserved Domain Database (CDD), GenBank (9), Genes and Disease, HomoloGene, Map Viewer, OMIM, PubMed, dbSNP (10), dbSTS and UniGene (11) (Table 2). In addition, nucleotide and protein sequences and OMIM records are linked to LocusLink reports via the NCBI LinkOut service. Following ‘LinkOut’ at the upper right side of an Entrez record is a convenient method to access a wealth of information about that gene. Links between LocusLink and Genes and Disease, HomoloGene, RefSeq, dbSNP and UniGene are based on shared identifiers stored by these resources (e.g., MIM numbers or GenBank accessions). Links to dbSTS and CDD are computed based on electronic PCR (ePCR) (12) and BLAST, respectively. And links to the NCBI Map Viewer are based on sequence identity, cytogenetic location or genetic map data. URLs to these resources are provided in Table 3.
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Several organisms and features have been added to the LocusLink web site over the past year and are listed below. LocusLink provides links to each resource whenever additional information is available.
Conserved domains: CDD domains annotated on RefSeq proteins are summarized in the product section of LocusLink reports. This section also includes the protein name, alternate names and enzyme commission (EC) numbers.
GeneRIF: LocusLink now supports community-generated functional annotation. LocusLink reports display submitted GeneRIFs (Gene References Into Function) and include a link to the submission form. Submissions consist of a publication (PubMed ID) and brief comment summarizing the functional information supported by the publication.
HomoloGene: HomoloGene reports UniGene clusters that are either calculated to be homologous, based on sequence identity, or reported to be homologous. HomoloGene also reports the ‘best’ homologous sequence pairs. HomoloGene includes all genomes in UniGene (human, mouse, rat and zebrafish), as well as Drosophila melanogaster mRNAs.
Maps: LocusLink now provides links to the Map Viewer and the Human/Mouse Homology map, as appropriate. The Map Viewer presents graphical views of various maps at the whole genome and single chromosome levels. This resource is currently available for D.melanogaster, Homo sapiens and Mus musculus. Views include presentation of genes, STS markers, SNPs and disease phenotypes along the chromosomes. The Human/Mouse Homology maps display genes, thought to be orthologous, ordered by MGD’s genetic map and the human sequence map.
Organisms: LocusLink now includes fruit fly, human, mouse, rat and zebrafish genes. The query interface supports retrieving results for all species, restricting to a single organism and displaying query results as a ‘Brief’ or expanded ‘Summary’ display.
Reference sequences: The RefSeq section of the LocusLink report has been expanded to include mitochondrial records, genomic contigs and modeled transcripts and proteins produced by the NCBI Human Genome Annotation effort. RefSeq data for a locus may include curated RefSeq records representing known transcripts, a genomic contig and modeled transcripts that overlap, but are distinct from, the known transcripts. Table 1 indicates the different accession number formats and categories they represent.
RefSeq
RefSeq transcript and corresponding protein records are now produced through two independent processes. The first process, which relies heavily on manual curation, provides a non-redundant dataset of transcripts and proteins of known genes for human, mouse and rat; these ‘known’ genes have some supporting evidence for the existence of the gene although the protein function may not be clear. The second process is NCBI’s Human Genome Annotation project, which will be described in detail elsewhere. Briefly, working draft and finished human genome sequence is assembled into contigs (13); gene predictions methods are used to produce the modeled mRNA and protein RefSeq records (see Table 1). The modeled transcripts and proteins may have different degrees of mRNA or EST evidence, or may be predicted ab initio.
RefSeq records include links to LocusLink where additional information is often available. The organisms and features added to RefSeq records over the past year are summarized below.
Category: RefSeq mRNA and protein records are now provided in four categories (previously termed ‘Status’ in LocusLink reports): (i) genome annotation, (ii) predicted, (iii) provisional and (iv) reviewed. The genome annotation category includes contigs, modeled mRNAs and corresponding modeled proteins. Predicted records represent genes of unknown function that are supported by mRNA sequence generated in a full-length insert cDNA sequencing project, homology or ESTs; the location of the protein product may be predicted in these records. Provisional and reviewed RefSeq records largely represent genes with known or inferred function. Provisional RefSeqs are not yet reviewed, whereas reviewed RefSeq records have been individually reviewed by NCBI staff and are richly annotated. Reviewed records include publications, a gene description and annotation that may not be on the provisional RefSeq or original GenBank records. Furthermore the sequence may be modified, relative to the source GenBank sequence, to extend the UTR, provide a transcript variant or remove contaminating vector or linker sequence. By including sequence data derived from more than one GenBank record and from the literature, reviewed RefSeq records may provide a full-length mRNA sequence that is not available in a single GenBank record.
Conserved domains: BLAST analysis is used to compare RefSeq proteins to the NCBI CDD; significant domain matches are annotated on the protein record and are linked to the CDD resource where additional information is available. Reviewed RefSeq proteins may include additional domain annotation derived from the literature.
Links: RefSeq records include links that facilitate access to additional information. Links are provided to: (i) the record(s) from which the mRNA (and hence protein) was derived, (ii) LocusLink, (iii) OMIM, (iv) MGD, (v) RGD and (vi) CDD, as appropriate. In addition, an overview of sequences that share sequence identity is available through the ‘Related sequences’ link at the top right of RefSeq (and GenBank) records.
Organisms: The mRNA/protein RefSeq resource now provides sequence standards for human, mouse and rat. Review of all three organisms to verify the initial ‘gene name-to-sequence’ association and to provide highly annotated reviewed RefSeq records is ongoing.
Variation: Variation features are automatically added to Genome Annotation RefSeq records using the data available in dbSNP. This expanded annotation will also be added to RefSeq records representing known genes.
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GROWTH AND MAINTENANCE |
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TOPABSTRACTBACKGROUNDEXPANDED AND NEW FEATURESGROWTH AND MAINTENANCEREFERENCES |
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The information available in LocusLink and RefSeq is continually being reviewed and augmented. As illustrated in Figure 1, the number of RefSeq records tripled, and LocusLink expanded ~5-fold over a 1-year period. New or updated RefSeq records are made publicly available on a daily basis. The LocusLink web site is updated weekly; a subset of data on the FTP site updated daily and the remainder is updated weekly.
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FOOTNOTES |
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* To whom correspondence should be addressed. Tel: +1 301 435 5898; Fax: +1 301 480 2290; Email: pruitt{at}ncbi.nlm.nih.gov
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J. M. Cameron, T. Hurd, and B. H. Robinson Computational identification of human mitochondrial proteins based on homology to yeast mitochondrially targeted proteins Bioinformatics, May 1, 2005; 21(9): 1825 - 1830. [Abstract] [Full Text] [PDF]
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M. Nakao, R. A. Barrero, Y. Mukai, C. Motono, M. Suwa, and K. Nakai Large-scale analysis of human alternative protein isoforms: pattern classification and correlation with subcellular localization signals Nucleic Acids Res., April 28, 2005; 33(8): 2355 - 2363. [Abstract] [Full Text] [PDF]
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P. M. Harrison, D. Zheng, Z. Zhang, N. Carriero, and M. Gerstein Transcribed processed pseudogenes in the human genome: an intermediate form of expressed retrosequence lacking protein-coding ability Nucleic Acids Res., April 28, 2005; 33(8): 2374 - 2383. [Abstract] [Full Text] [PDF]
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K. Wolstencroft, R. McEntire, R. Stevens, L. Tabernero, and A. Brass Constructing ontology-driven protein family databases Bioinformatics, April 15, 2005; 21(8): 1685 - 1692. [Abstract] [Full Text] [PDF]
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R. Castelo, A. Reymond, C. Wyss, F. Camara, G. Parra, S. E. Antonarakis, R. Guigo, and E. Eyras Comparative gene finding in chicken indicates that we are closing in on the set of multi-exonic widely expressed human genes Nucleic Acids Res., April 4, 2005; 33(6): 1935 - 1939. [Abstract] [Full Text] [PDF]
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X. Zhang, D. T. Odom, S.-H. Koo, M. D. Conkright, G. Canettieri, J. Best, H. Chen, R. Jenner, E. Herbolsheimer, E. Jacobsen, et al. Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues PNAS, March 22, 2005; 102(12): 4459 - 4464. [Abstract] [Full Text] [PDF]
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S. W. Cole, W. Yan, Z. Galic, J. Arevalo, and J. A. Zack Expression-based monitoring of transcription factor activity: the TELiS database Bioinformatics, March 15, 2005; 21(6): 803 - 810. [Abstract] [Full Text] [PDF]
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J. Yan and T. G. Marr Computational analysis of 3'-ends of ESTs shows four classes of alternative polyadenylation in human, mouse, and rat Genome Res., March 1, 2005; 15(3): 369 - 375. [Abstract] [Full Text] [PDF]
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J. Kim, J. Seo, Y. S. Lee, and S. Kim TFExplorer: integrated analysis database for predicted transcription regulatory elements Bioinformatics, February 15, 2005; 21(4): 548 - 550. [Abstract] [Full Text] [PDF]
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K. Tan, L. A. McCue, and G. D. Stormo Making connections between novel transcription factors and their DNA motifs Genome Res., February 1, 2005; 15(2): 312 - 320. [Abstract] [Full Text] [PDF]
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A. Kahraman, A. Avramov, L. G. Nashev, D. Popov, R. Ternes, H.-D. Pohlenz, and B. Weiss PhenomicDB: a multi-species genotype/phenotype database for comparative phenomics Bioinformatics, February 1, 2005; 21(3): 418 - 420. [Abstract] [Full Text] [PDF]
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L. A. Blomberg, E. L. Long, T. S. Sonstegard, C. P. Van Tassell, J. R. Dobrinsky, and K. A. Zuelke Serial analysis of gene expression during elongation of the peri-implantation porcine trophectoderm (conceptus) Physiol Genomics, January 20, 2005; 20(2): 188 - 194. [Abstract] [Full Text] [PDF]
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L. Chen, H. Liu, and C. Friedman Gene name ambiguity of eukaryotic nomenclatures Bioinformatics, January 15, 2005; 21(2): 248 - 256. [Abstract] [Full Text] [PDF]
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L. Florea, V. Di Francesco, J. Miller, R. Turner, A. Yao, M. Harris, B. Walenz, C. Mobarry, G. V. Merkulov, R. Charlab, et al. Gene and alternative splicing annotation with AIR Genome Res., January 1, 2005; 15(1): 54 - 66. [Abstract] [Full Text] [PDF]
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S. J. Hubbard, D. V. Grafham, K. J. Beattie, I. M. Overton, S. R. McLaren, M. D.R. Croning, P. E. Boardman, J. K. Bonfield, J. Burnside, R. M. Davies, et al. Transcriptome analysis for the chicken based on 19,626 finished cDNA sequences and 485,337 expressed sequence tags Genome Res., January 1, 2005; 15(1): 174 - 183. [Abstract] [Full Text] [PDF]
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H. Zhang, J. Hu, M. Recce, and B. Tian PolyA_DB: a database for mammalian mRNA polyadenylation Nucleic Acids Res., January 1, 2005; 33(suppl_1): D116 - D120. [Abstract] [Full Text] [PDF]
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A. Bairoch, R. Apweiler, C. H. Wu, W. C. Barker, B. Boeckmann, S. Ferro, E. Gasteiger, H. Huang, R. Lopez, M. Magrane, et al. The Universal Protein Resource (UniProt) Nucleic Acids Res., January 1, 2005; 33(suppl_1): D154 - D159. [Abstract] [Full Text] [PDF]
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Y. Chen, Y. Zhang, Y. Yin, G. Gao, S. Li, Y. Jiang, X. Gu, and J. Luo SPD--a web-based secreted protein database Nucleic Acids Res., January 1, 2005; 33(suppl_1): D169 - D173. [Abstract] [Full Text] [PDF]
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V. Giudicelli, D. Chaume, and M.-P. Lefranc IMGT/GENE-DB: a comprehensive database for human and mouse immunoglobulin and T cell receptor genes Nucleic Acids Res., January 1, 2005; 33(suppl_1): D256 - D261. [Abstract] [Full Text] [PDF]
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H. Mi, B. Lazareva-Ulitsky, R. Loo, A. Kejariwal, J. Vandergriff, S. Rabkin, N. Guo, A. Muruganujan, O. Doremieux, M. J. Campbell, et al. The PANTHER database of protein families, subfamilies, functions and pathways Nucleic Acids Res., January 1, 2005; 33(suppl_1): D284 - D288. [Abstract] [Full Text] [PDF]
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G. L. Winsor, R. Lo, S. J. H. Sui, K. S.E. Ung, S. Huang, D. Cheng, W.-K. H. Ching, R. E. W. Hancock, and F. S. L. Brinkman Pseudomonas aeruginosa Genome Database and PseudoCAP: facilitating community-based, continually updated, genome annotation Nucleic Acids Res., January 1, 2005; 33(suppl_1): D338 - D343. [Abstract] [Full Text] [PDF]
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H. J. Kang, K. O. Choi, B.-D. Kim, S. Kim, and Y. J. Kim FESD: a Functional Element SNPs Database in human Nucleic Acids Res., January 1, 2005; 33(suppl_1): D518 - D522. [Abstract] [Full Text] [PDF]
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M. Shklar, L. Strichman-Almashanu, O. Shmueli, M. Shmoish, M. Safran, and D. Lancet GeneTide--Terra Incognita Discovery Endeavor: a new transcriptome focused member of the GeneCards/GeneNote suite of databases Nucleic Acids Res., January 1, 2005; 33(suppl_1): D556 - D561. [Abstract] [Full Text] [PDF]
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R. Homayouni, K. Heinrich, L. Wei, and M. W. Berry Gene clustering by Latent Semantic Indexing of MEDLINE abstracts Bioinformatics, January 1, 2005; 21(1): 104 - 115. [Abstract] [Full Text] [PDF]
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H. Iwama and T. Gojobori Highly conserved upstream sequences for transcription factor genes and implications for the regulatory network PNAS, December 7, 2004; 101(49): 17156 - 17161. [Abstract] [Full Text] [PDF]
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L. Ding, A. Sabo, N. Berkowicz, R. R. Meyer, Y. Shotland, M. R. Johnson, K. H. Pepin, R. K. Wilson, and J. Spieth EAnnot: A genome annotation tool using experimental evidence Genome Res., December 1, 2004; 14(12): 2503 - 2509. [Abstract] [Full Text] [PDF]
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L. Zhang, S. Kasif, C. R. Cantor, and N. E. Broude GC/AT-content spikes as genomic punctuation marks PNAS, November 30, 2004; 101(48): 16855 - 16860. [Abstract] [Full Text] [PDF]
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P. D. Thomas and A. Kejariwal Coding single-nucleotide polymorphisms associated with complex vs. Mendelian disease: Evolutionary evidence for differences in molecular effects PNAS, October 26, 2004; 101(43): 15398 - 15403. [Abstract] [Full Text] [PDF]
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W. Thompson, M. J. Palumbo, W. W. Wasserman, J. S. Liu, and C. E. Lawrence Decoding Human Regulatory Circuits Genome Res., October 1, 2004; 14(10a): 1967 - 1974. [Abstract] [Full Text] [PDF]
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T. S. Furey, M. Diekhans, Y. Lu, T. A. Graves, L. Oddy, J. Randall-Maher, L. W. Hillier, R. K. Wilson, and D. Haussler Analysis of Human mRNAs With the Reference Genome Sequence Reveals Potential Errors, Polymorphisms, and RNA Editing Genome Res., October 1, 2004; 14(10b): 2034 - 2040. [Abstract] [Full Text] [PDF]
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D. N. Messina, J. Glasscock, W. Gish, and M. Lovett An ORFeome-based Analysis of Human Transcription Factor Genes and the Construction of a Microarray to Interrogate Their Expression Genome Res., October 1, 2004; 14(10b): 2041 - 2047. [Abstract] [Full Text] [PDF]
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M. Oyama, C. Itagaki, H. Hata, Y. Suzuki, T. Izumi, T. Natsume, T. Isobe, and S. Sugano Analysis of Small Human Proteins Reveals the Translation of Upstream Open Reading Frames of mRNAs Genome Res., October 1, 2004; 14(10b): 2048 - 2052. [Abstract] [Full Text] [PDF]
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The MGC Project Team The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) Genome Res., October 1, 2004; 14(10b): 2121 - 2127. [Abstract] [Full Text] [PDF]
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B. Issac and G. P. S. Raghava EGPred: Prediction of Eukaryotic Genes Using Ab Initio Methods After Combining With Sequence Similarity Approaches Genome Res., September 1, 2004; 14(9): 1756 - 1766. [Abstract] [Full Text] [PDF]
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B. H. Mecham, D. Z. Wetmore, Z. Szallasi, Y. Sadovsky, I. Kohane, and T. J. Mariani Increased measurement accuracy for sequence-verified microarray probes Physiol Genomics, August 11, 2004; 18(3): 308 - 315. [Abstract] [Full Text] [PDF]
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J. Leipzig, P. Pevzner, and S. Heber The Alternative Splicing Gallery (ASG): bridging the gap between genome and transcriptome Nucleic Acids Res., August 3, 2004; 32(13): 3977 - 3983. [Abstract] [Full Text] [PDF]
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G. Rajashekara, J. D. Glasner, D. A. Glover, and G. A. Splitter Comparative Whole-Genome Hybridization Reveals Genomic Islands in Brucella Species J. Bacteriol., August 1, 2004; 186(15): 5040 - 5051. [Abstract] [Full Text] [PDF]
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M. R. Nelson, G. Marnellos, S. Kammerer, C. R. Hoyal, M. M. Shi, C. R. Cantor, and A. Braun Large-Scale Validation of Single Nucleotide Polymorphisms in Gene Regions Genome Res., August 1, 2004; 14(8): 1664 - 1668. [Abstract] [Full Text] [PDF]
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P. C. FitzGerald, A. Shlyakhtenko, A. A. Mir, and C. Vinson Clustering of DNA Sequences in Human Promoters Genome Res., August 1, 2004; 14(8): 1562 - 1574. [Abstract] [Full Text] [PDF]
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P. M. Haverty, M. C. Frith, and Z. Weng CARRIE web service: automated transcriptional regulatory network inference and interactive analysis Nucleic Acids Res., July 1, 2004; 32(suppl_2): W213 - W216. [Abstract] [Full Text] [PDF]
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V. B. Vega, D. K. Bangarusamy, L. D. Miller, E. T. Liu, and C.-Y. Lin BEARR: Batch Extraction and Analysis of cis-Regulatory Regions Nucleic Acids Res., July 1, 2004; 32(suppl_2): W257 - W260. [Abstract] [Full Text] [PDF]
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