Nucleic Acids Research, 2001, Vol. 29, No. 1 178-180
© 2001 Oxford University Press
RISSC: a novel database for ribosomal 16S–23S RNA genes spacer regions
División de Microbiología, Campus de San Juan and 1Centro de Cálculo, Edificio de La Gal·lia, Universidad Miguel Hernández, Elche, Alicante, Spain
Received July 6, 2000; Revised and Accepted September 6, 2000.
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ABSTRACT |
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 TOP ABSTRACT DESCRIPTION OF THE DATABASEUSING RISSCREFERENCES |
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A novel database, under the acronym RISSC (Ribosomal Intergenic Spacer Sequence Collection), has been created. It compiles more than 1600 entries of edited DNA sequence data from the 16S–23S ribosomal spacers present in most prokaryotes and organelles (e.g. mitochondria and chloroplasts) and is accessible through the Internet (http://ulises.umh.es/RISSC), where systematic searches for specific words can be conducted, as well as BLAST-type sequence searches. Additionally, a characteristic feature of this region, the presence/absence and nature of tRNA genes within the spacer, is included in all the entries, even when not previously indicated in the original database. All these combined features could provide a useful documentation tool for studies on evolution, identification, typing and strain characterization, among others.
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DESCRIPTION OF THE DATABASE |
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TOPABSTRACTDESCRIPTION OF THE DATABASEUSING RISSCREFERENCES |
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The development of free-access Molecular Biology databases via the Internet has increased dramatically over the past few years as the scientific community has become more familiar with this technological tool (1–3). Their utility is evident, even for the simplest databases, since they provide easy and fast access to a variety of relevant information that would otherwise be tedious and time-consuming to obtain.
One major issue in microbiology is the identification of microorganisms, even more so recently due to rekindling of prokaryotic biodiversity studies. Commonly, molecular identification techniques involve the sequencing of the prokaryotic 16S rRNA gene. This has proved to be useful in establishing coherent phylogenetic relationships at the taxonomic level of species or higher (genus, family, etc.) (4,5), but it often lacks accuracy in discriminating strains within the same species and sometimes even different species within the same genus (6). In contrast, the internal transcribed spacers (ITS), located between the 16S and 23S genes in most prokaryotic ribosomal RNA operons, are much more variable than the adjacent 16S and 23S ribosomal genes. Typically, this region consists of a series of conserved, alignable DNA stretches found in all strains of a single species, but rarely beyond the genus or family level, combined with hypervariable DNA segments (in the form of insertions, deletions and/or highly variable sequences of equal length) (7,8). The alignable stretches can be used for very precise species identification while the hypervariable stretches often allow strain characterization. These regions may vary even among the different operons within a single cell (intercistronic heterogeneity), particularly when several operons are present (9). The location of the ITS flanked by the highly conserved 16S and 23S rRNA genes allows for very easy PCR amplification using universal primers. In fact sequencing, or characterization by other means, of the ITS has become rather common over the past years for typing work in population genetics or molecular epidemiology (7,10–12).
From the previous argument it is easy to see the usefulness of a spacer database for fine species and/or strain characterization of Bacteria and Archaea. However, many of these sequences deposited in the EMBL/GenBank/DDBJ and other databases were not originally intended for these kinds of studies and they are very often submitted as part of a complete ribosomal operon sequence. Even when deposited as spacers, they may have partial 16S and/or 23S sequences attached, not even being characterized as such. Frequently, an ITS with flanking 16S/23S gene sequences may cause inconvenience when carrying out BLAST searches (since ribosomal homology often yields unwanted ‘best’ matches) or in experiments such as species or strain-specific primer design from aligned sequences. Moreover, one of the most conserved parts of the ITS, if present, the tRNA gene, also of great importance in typing and evolution experiments (13,14), is not always reported. Being highly conserved, the tRNA section can also bias BLAST searches. At RISSC we have carried out editing and tRNA searching of the 16S–23S spacers available at other databases, for better characterization.
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USING RISSC |
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TOPABSTRACTDESCRIPTION OF THE DATABASEUSING RISSCREFERENCES |
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Upon entering the RISSC web page at http://ulises.umh.es/RISSC, visitors will find detailed instructions about how to proceed. Commands are presented in a similar way to other known databases to facilitate straightforward use. Among others, BLAST-type searches (15) can be conducted by downloading the appropriate program and obtaining the database of spacers cured from 16S and/or 23S tails (Fig. 1). The SIZE option is designed to delimitate a size range for a specific search. The ITS may vary greatly in length, from few bases to almost a kilobase and more (Fig. 2). The presence/absence of tRNA genes, as indicated by the tRNAscan-SE v1.11 program (http://www.genetics.wustl.edu/eddy/tRNAscan-SE) (16), is shown in the FEATURES field as well as their arrangement within the spacer. tRNA genes are important as phylogenetic markers, since their presence or absence in the ITS is characteristic of certain groups of prokaryotes (7,8,13). Nevertheless, almost 40% (302 sequences out of 790, August 30, 2000) of all these genes recorded at RISSC had previously gone unreported. Observations on the spacer sequence distribution according to the contributions of different phylogenetic groups also indicates that some groups are poorly represented (or not represented at all), with preference (in terms of number of entries) for those microorganism species of importance in clinical and applied microbiology (Table 1). This should be taken as an encouragement to scientists to expand their studies and shows how much work has yet to be done.
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subdivision; 6, ß subdivision; 7, 
subdivision; 8, 
/
subdivision; 9, Cytophaga/Flexibacter/Bacteroides; 10, Thermotogales; 11, Aquificales; 12, unidentified; 13, cyanobacteria; 14, chloroplasts; 15, Euryarchaeota; 16, Crenarchaeota. *, plastids.
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ACKNOWLEDGEMENTS |
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We thank I. Jarrín for her assistance in some of the statistical analyses. I.B. is holder of a doctoral grant from the Spanish Ministry of Culture, J.J.R.-S. is an IMPIVA grant holder, J.G.-M. is the recipient of a postdoctoral European Commission fellowship MAS3-CT-97-0154, UMH.DCET.DM.B, MIDAS project.
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FOOTNOTES |
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* To whom correspondence should be addressed at: División de Microbiología, Universidad Miguel Hernández, Campus de San Juan, Carretera de Valencia Km 87, Apartado 18, 03550 San Juan, Alicante, Spain. Tel: +34 965 919 451; Fax: +34 965 919 457; Email: frvalera{at}umh.es
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REFERENCES |
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TOPABSTRACTDESCRIPTION OF THE DATABASEUSING RISSCREFERENCES |
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