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Figure 1. The reporter plasmids used in this study. (A) Structure of the plasmids. The symbols ‘mcs’ and ‘luc’ indicate the multiple cloning site and the luciferase gene, respectively. Positions of the (T/A)5 tracts are indicated with bold bars. The deleted nucleotides in the mcs are indicated by lines. The nucleotides inserted into the mcs are shaded. The centers of curved DNA and the TATA box are marked with asterisks. (B) Three-dimensional architecture of the curved and straight DNA segments in the plasmids. They were drawn by a combination of softwares DIAMOD (50) and RASMOL (51). The modeling algorithm was based on that of Bolshoy et al. (52) with the twist angles from Kabsch et al. (53). For left-handedly and right-handedly curved DNA structures, the superhelical axis is drawn.
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