Published online 25 February 2004
Nucleic Acids Research, 2004, Vol. 32, No. 4 1363-1371
© 2004 Oxford University Press
ARB: a software environment for sequence data
Lehrstuhl für Mikrobiologie, Technische Universität München, D-853530 Freising, Germany 1 Lehrstuhl für Rechnertechnik und Rechnerorganisation, Parallelrechnerarchitektur, Technische Universität München, D-85748 Garching, Germany 2 Institut für Informatik, Ruprecht-Karls-Universität Heidelberg, D-69120 Heidelberg, Germany
*To whom correspondence should be addressed. Tel: +8161 71 5451; Fax: +8161 71 5475; Email: ludwig{at}mikro.biologie.tu-muenchen.de
Received January 13, 2004; Revised and Accepted January 28, 2004
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTS AND DISCUSSIONREFERENCES |
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The ARB (from Latin arbor, tree) project was initiated almost 10 years ago. The ARB program package comprises a variety of directly interacting software tools for sequence database maintenance and analysis which are controlled by a common graphical user interface. Although it was initially designed for ribosomal RNA data, it can be used for any nucleic and amino acid sequence data as well. A central database contains processed (aligned) primary structure data. Any additional descriptive data can be stored in database fields assigned to the individual sequences or linked via local or worldwide networks. A phylogenetic tree visualized in the main window can be used for data access and visualization. The package comprises additional tools for data import and export, sequence alignment, primary and secondary structure editing, profile and filter calculation, phylogenetic analyses, specific hybridization probe design and evaluation and other components for data analysis. Currently, the package is used by numerous working groups worldwide.
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTS AND DISCUSSIONREFERENCES |
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The ARB (from Latin arbor, tree) project was established as an interdisciplinary bioinformatics initiative of the Lehrstuhl für Mikrobiologie and the Lehrstuhl für Rechnertechnik und Rechnerorganisation, Parallelrechnerarchitektur of the Technical University of Munich almost 10 years ago. In that time, comparative sequence analysis of the small subunit rRNAs or the respective genes had already been established as the most commonly applied approach for phylogeny inference as well as microbial taxonomy and identification. Further more, improved and automated sequencing techniques promoted a rapid increase in the number of small subunit rRNA primary structure data available from data sources such as GenBank (1) or EBI (European Bioinformatics Institute) (2). However, these databases provide only raw data and additional descriptive information which cannot interactively be extended by the user. Although the Ribosomal Database Project (RDP) (3) and the Antwerpen projects (4,5) offered datasets of aligned sequences, data handling and analysis remained difficult for scientists applying rRNA-based methods. A variety of individual software tools for sequence editing, alignment and phylogenetic analysis were available from the different database projects (1–4) and other sources (6) (http://www.gcg.com). However, a comprehensive package of interacting tools was missing. Furthermore, the number of different input and output formats which had to be used reflected the variety of individual software programs which uncomfortably had to be applied sequentially to achieve a comprehensive analysis of molecular data. Unfortunately, a promising initiative, the Genetic Data Environment (GDE) project (http://bimas.dcrt.nih.gov/gde_sw.html), focusing on the development of a common graphical interface for data handling and analysis was not continued. Consequently, microbiologists and computer scientists at the Technical University of Munich decided to develop their own software package capable of properly managing the upcoming data flood.
The two major tasks according to the ARB concept, formulated in the early days of the project and maintained to the present, are (i) the maintenance of a structured integrative secondary database combining processed primary structures and any type of additional data assigned to the individual sequence entries and (ii) a comprehensive selection of software tools directly interacting with one another as well as the central database which are controlled via a common graphical interface. Software and rRNA databases are publicly accessible (http://www.arb-home.de) and have been in use worldwide for several years.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTS AND DISCUSSIONREFERENCES |
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Sequence data
The raw data used to establish databases and perform data analysis were taken from our own sequencing projects, provided by other research groups or periodically retrieved from public data sources such as the EBI (1), Genbank (2), the RDP (3) and the Antwerpen databases for small (4) and large (5) subunit RNAs. Complete releases were downloaded from the latter two locations. The search and retrieval tools of the former two institutions were used to select and download the primary structure and additional information on rRNA or other genes. Furthermore, sequence data determined at the Lehrstuhl für Mikrobiologie of the Technical University of Munich or by other groups were imported and processed.
Operating systems and programming languages
The ARB software was developed for UNIX systems and their derivatives. Currently, the development is performed using SuSE LINUX (http://www.suse.com) running on PCs.
The greater part of the source code was written in C++ and C; some parts were written in Perl and other script languages. The graphical environment is based upon the Open Motif library.
Integrated external software tools
Functionalities from the GDE project (http://bimas.dcrt.nih. gov/gde_sw.html) concerning sequence editing were adopted and implemented in the ARB package. Some programs of the PHYLIP package for phylogeny inference (6) were incorporated as components directly interacting with the central database. Additionally, fastDNAml (7) and protml of the Molphy package (8), components of the Puzzle package (9) and AxML, a new accelerated fastDNAml derivative (10), were included for maximum-likelihood-based phylogenetic analyses of nucleic and amino acid sequence data.
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RESULTS AND DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTS AND DISCUSSIONREFERENCES |
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A selection of tools and functionalities of the ARB packages will be briefly described in the following sections. The network in Figure 1 schematically visualizes these tools and their interactions with one another and the central database. Most tools developed for ARB directly interact with a copy of the database in the main storage, whereas the integrated second-party tools are provided with data from ARB and their results are written back to the database. Thus any changes or rearrangements are immediately known to the peripheral software components.
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The central database
The sequences representing organisms, genes or gene products are stored in individual database fields as the central components and a unique identifier (short_name) is automatically generated and assigned to each of them. Databases created using ARB are hierarchically structured. Following the ARB concept of an integrative database, any type of additional data can be assigned to the individual sequence data entry and stored within default or user-defined database fields. These data can either be kept as intrinsic components of the database or linked to it via local networks or the Internet. In the latter case the path to the respective file or the URL of an external database, optionally including commands and search strings, have to be stored within the respective ARB database fields. The designations and hierarchy of the database fields can be customized by the user. The default structuring is according to the phylogeny of the organisms derived from the respective sequence data. However, it can also be changed according to other criteria defined by database field entries. This hierarchy is used by special algorithms for highly effective data compression. Different protection levels (0–6) can be assigned to the individual database fields. Database as well as security management is facilitated by this tool.
Data access and visualization
A powerful search tool allows simple (strings and combination of strings) and complex (default or user-defined algorithms) searches in one or more of the database fields. The information in all or a user-defined selection of database fields can be visualized on the screen in respective windows (Fig. 2). The layout of the visualization windows, i.e. selection, size and positioning of database field entries, can be customized by the user. Simple algorithms are included.
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An alternative method of data access and visualization is provided by the ARB main window. Phylogenetic trees generated by intrinsic ARB tree reconstruction tools or imported from external sources are stored in the database and can be visualized in different formats within the ARB main window (Fig. 3). Any (combination of) database field entries can be visualized at the terminal nodes of the tree currently shown. Selection and order of data entries, the results of data analysis or extractions to be visualized are defined by the node display settings (NDS) tool. Irrespective of the visualization mode used, the ARB search and replacement tool (SRT) and ARB command interpreter (ACI) can be used for extraction of combinations of (sub)strings as well as for analysis of database field entries, respectively.
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Sequence editors
The sequence data can be visualized and modified with a powerful editor (Fig. 4). The original data as well as virtually transformed data (e.g. purine–pyrimidine or simplified amino acid presentation) are displayed in user-defined color codes. Keyboard customization is possible for data entry and modification. Two different editing modes can be selected. The ‘Align’ mode allows only insertion/removal of alignment gaps and movement of sequence characters. In addition to these functions, character changes can be performed in the ‘Edit’ mode. The rights to overcome protection of the individual sequence entries can be given for the two modes independently. This helps to prevent unwanted character changes when manually modifying the sequence data or alignment.
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Sets of search strings can be defined and optionally stored. Their occurrence can be visualized within the displayed sequences by user-defined background colors. Virtual compression (removal of alignment gaps common to all or a certain fraction of the displayed sequences) is possible. This makes data handling more convenient in the case of large insertions occurring in only part of the selected sequences. Groups of sequences can be interactively defined or are automatically shown if defined in the phylogenetic trees. Consensus sequences are determined for each defined group of sequences according to default or user-defined criteria and optionally visualized along with or instead of the individual sequences. This consensus can be edited and changes made concern any sequence in the group. A special feature of the editor is the simultaneous secondary structure check if rRNA (gene) data are visualized. Symbols indicating the presence or absence as well as the character of base pairing are shown below the individual characters and immediately refreshed during sequence editing. A (three-domain) consensus secondary structure mask established according to commonly accepted secondary structure models (11) functions as a guide for this tool. Thereby the users are strongly supported with regard to the evaluation of sequences, alignment and probe targets.
The ARB secondary structure editor (Fig. 5) fits any sequence into the common consensus model. The particular sequence to be visualized is selected by cursor positioning in the primary structure editor. The layout of the structure, i.e. color coding of base-paired, non-paired and loop positions as well as probe target sites, can be customized according to the user’s preferences. Any of the search strings activated in the primary structure editor can be indicated in the secondary structure model. This helps the experts to evaluate probe targets. The evaluation of target position with respect to higher-order rRNA structure is of importance especially when probes are used for in situ cell hybridizations (12–14). The structure can be exported to xfig, a simple open-source graphics program (http://ww.xfig.org), for further modification and/or transformation into various formats.
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Profiles, masks and filters
Conservation or base composition profiles, higher-order structure masks and filters including or excluding particular alignment positions are important tools for sequence data analyses, especially for phylogenetic inference (15). The ARB package provides tools for determining such profiles based upon the full database or user-defined subsets. The underlying methods range from simple character counting to maximum parsimony-based column statistics. These profiles, masks and filters are stored in the central database as so-called sequence associated information (SAI) and can be visualized and modified by the primary structure editor. The filter selection tool allows not only choice of sets of particular filters but also performance of fine tuning with respect to the inclusion or exclusion of alignment positions in the case of multiple character filters.
Phylogenetic treeing
As mentioned in Materials and Methods, software implementations of several alternative treeing methods are incorporated in the package. They operate as intrinsic tools with all the respective ARB components and database elements such as alignment and filters. The central treeing tool of the package, ARB-parsimony, is a special development for the handling of several thousand sequences (more than 30 000 in the current small subunit rRNA ARB database). New sequences are successively added to an existing tree according to the parsimony criterion. An intrinsic software component superimposes branch length on the parsimony-generated tree topology. These branch lengths reflect the significance of the individual ‘tetra-furcations’ by expressing the difference of the most parsimonious and the two least parsimonious solutions when performing nearest-neighbor interchange (NNI) of adjacent branches or subtrees. These relative distances are standardized according to a distance matrix deduced from primary structure comparison. Thus branch lengths in ARB-parsimony generated trees in the first instance visualize the significance of topologies, and in the second instance reflect a degree of estimated sequence divergence. A prominent feature of ARB-parsimony is the possibility of adding sequences to an existing tree without allowing any changes in the initial tree. This enables the user to reconstruct and optimize an initial tree based upon the best (full sequences) and most comprehensive (wide variation of phylogenetic levels) sequence data and also to include partial sequences without perturbing the initial tree topology. The second peculiarity of the treeing software concerns the tree optimization performing cycles of NNI and Kernigham–Lin (KL) (16) tree modifications. This optimization can not only be applied to the complete tree but also confined to user-selected subtrees. Thus tree optimization is possible applying the appropriate filters for the respective phylogenetic levels and groups. In this context, it is of interest that, while performing stepwise optimizations, the intermediates are stored until the user defines the version to be permanently stored in the database. Furthermore, different trees generated applying various parameters can be permanently kept in the database and optionally used for data visualization in the ARB main window.
The positional tree server
The ARB positional tree (PT) server, once established, allows rapid finding of sequence identity or peculiarity. Thus it is the central tool for fast search of closest relatives for automated sequence alignment or to define diagnostic sequence stretches for primer and probe design. Establishing a positional tree server of any oligonucleotide sequence up to 20mers occurring in the underlying database and assignment of the individual oligonucleotides to the sequences or organisms containing them is the basis for these procedures. PT-server-based analyses do not rely upon aligned sequences. The PT server is not provided with the ARB program package or ARB database. It has to be established for the respective database locally. The PT server can be used by multiple users on the local machine or via network. The computing time for generating the respective files depends strongly on the size and structure of the individual database as well as the performance of the machine used. The advantages of these logarithmic algorithms over linear ones such as Blast (17) or Fasta (18) are effectiveness and rapidity.
Sequence alignment
As mentioned in Materials and Methods, for de novo generation of a nucleic or amino acid sequence alignment ClustalW (19) as implemented in the ARB package can be used. However, in most cases new sequence entries have to be integrated in an already existing database of aligned sequences. For this purpose the ARB fast aligner was developed and included. This aligner uses a (set of) selected aligned reference sequences as template(s) for rapid integration of a (set of) unaligned sequence(s). Individual entries, i.e. sequences or consensus defined by the user or automatically determined by PT-server-based search for most similar reference sequences, are used as the template.
In the case of protein coding nucleic acid sequences the alignment is usually optimized on the amino acid level. The underlying nucleic acid alignment can then be adapted to the amino acid alignment by a back-translation-based tool taking into consideration all known codon usages.
Probe design and evaluation
Currently, taxon- or gene-specific probe design certainly plays a central role in many molecular biological research and analysis projects, for example the identification and detection of organisms in complex environmental samples or expression studies within the scope of genome projects. Algorithms of the ARB programs ‘Probe Design’ and ‘Probe Match’ are searching the PT server to identify short (10–100 monomers) diagnostic sequence stretches which are evaluated against the background of all full and partial sequences in the respective database the PT server has been built from. In principle, no alignment of the sequence data is needed for specific probe design. However, in the case of taxon-specific probes alignment and phylogenetic analyses are necessary to allow defining groups of phylogenetically (taxonomically) related organisms as the targets of specific probes. The design of taxon-specific oligonucleotide probes with ARB is performed in three steps. First, the user selects the organism or a group of organisms for which he or she wants to design a diagnostic probe. Secondly, the software ‘Probe Design’ searches the PT server for potential target sites. The results are shown in a ranked list of proposed targets, probes and additional information. The ranking is according to several compositional and thermodynamic criteria (12,20). Thirdly, the proposed oligonucleotide probes are evaluated against the whole database by using the program ‘Probe Match’. Local alignments are determined between the probe target sequence(s) and the most similar reference sequences (optionally from no to five mismatches) in the respective database (Fig. 6). Furthermore, these sequence strings can automatically be visualized in the primary and secondary structure editors. The latter information is of particular importance when designing probes for in situ cell hybridization. A tool for visualization of accessibility maps (13,14) in the primary and secondary structure editors is under development.
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A special advance is the ARB multiprobe software component. It determines sets of up to five probes optimally identifying the target group (21). These probe sets can be used for multiple fluorescence in situ hybridization experiments.
Data import and export
The sequence as well as additional data can be imported and exported in commonly used flat file formats. The parsing from and to tagged flat files can be customized by advanced users. There is also a tool for automated completion of database submission forms for those users determining sequences on their own.
Availability and documentation
Although so far not officially published, previous versions of the software package and databases have been available for several years and the software has been used worldwide. The ARB package provides a comprehensive set of tools to support the user’s work. However, depending upon the user’s interests, some knowledge of the basics of sequence alignment, phylogenetic analyses or probe hybridization is needed. Some familiarity with UNIX operating systems is advantageous. During installation, environment variables, paths, permissions and aliases have to be defined. Instructions for installation can be downloaded from http://www.arb-home.de/download/ARB/documentation/ARB_install.pdf. Self-installing versions of the recent program releases are currently available for Linux systems only. The binaries, source code and some documentation are available at the download area of the ARB web site http://www.arb-home.de/download/. An HTTP Browser is required as ftp connection is not accepted. Furthermore, there is an email forum of the worldwide ARB users community. Subscription is needed for those interested in joining (subscribe{at}arb-home.de). Although a comprehensive formal handbook is not yet available, manuals, instructions and problem solutions are available from the ARB homepage and by contacting the ARB staff and user community via the email forum. ARB sequence databases are currently available for small subunit rRNA, and those for other conserved genes will be provided soon. Checking for new releases and updates should be done at http:www. arb-home.de/downloads/databases/.
Systems, hardware and processing time requirements
The ARB group provides tested versions for SuSE LINUX and Sun Solaris systems. According to information provided by users, the LINUX version also runs on Redhat and Mandrake LINUX systems. For running ARB on Mac OSX see http://www.microbiol.unimelb.edu.au/micro/staff/mds/ARB_OSX/ARB_to_MacOSX.html. With respect to hardware requirements, mainframe memory is more important than processor performance. The users among the wet laboratory partners of the ARB group are performing their analyses on dual Pentium III PCs with 1 Gb memory and 1 Gb swap space. The background storage requirements depend mainly upon the number and size of the user ARB databases and PT servers. The sizes of the installed program package, the current small subunit rRNA database and the respective PT server files are about 25 Mb, 80 Mb and 350 Mb, respectively. Twenty-one-inch monitors at 1600 x 1200 are recommended. However, ARB is also routinely used on laptops or older PCs and workstations with less memory and monitors with lower resolution.
Table 1 gives some information on processing times for some of the major functions in ARB: generation of the PT server, automated sequence alignment and phylogenetic treeing. Run-time measurements were performed on a dual-processor (Intel® XeonTM, 2.6 GHz) PC equipped with a 2 Gb RAM running SuSE Linux 8.2. The ARB databases ssu_ jan03.arb (25 743 almost complete small subunit rRNA sequences, 40 000 alignment positions) and ssu_10k.arb, ssu_1k.arb, ssu_100.arb (subsets comprising 10 000, 1000 and 100 sequences) used for these PT server building studies are available at http://www.arb-home.de. For adding 10, 100 and 1000 sequences to the database alignment or tree, ssu_ jan03.arb was used as basis. The ARB aligner was used in combination with a PT-server-directed search for most similar reference sequences. For phylogenetic treeing the respective sequences/organisms were added to a tree comprising all entries of the database applying the ARB parsimony tool.
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Future developments
The ongoing developments are focusing on two major tasks. First, a web tool providing all potential probe target sites which can be derived from the current database version and should phylogenetically (taxonomically) make sense. Users can not only search for hierarchical and multiple probes submitting names, strain designations or accession numbers of organisms as search strings, but can also send their own probe sequences for in silico evaluation. Second, the package is adopted for handling and analysing databases of completed and annotated genomes. All ARB functionalities can be applied and genome maps can be used for visualization and data access. In accordance with the ARB concept of integrative databases experimental parameters and data can be stored and assigned to the individual genomes or genes.
Many users ask for a Windows-compatible ARB version. Although comprehensive software redesign would be desirable, the current capacity and funding of the ARB group does not allow doing this in reasonable time with a source code developed by many individual scientists and programmers.
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ACKNOWLEDGEMENTS |
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The authors highly acknowledge F. O. Glöckner and R. Amann (Max Planck Institute for Marine Microbiology, Bremen, Germany) for redistributing the ARB database and software and answering user queries, as well as hosting and organizing ARB workshops. The authors thank Laura Schulz (Ludwig Maximilian University, Munich, Germany) for critical reading of the manuscript. ARB software development and database maintenance was partly supported by the European Union within the HRAMI project, by the German Research Foundation and by the German Ministry of Research and Education BIOLOG project.
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REFERENCES |
|---|
|
TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTS AND DISCUSSIONREFERENCES |
|---|
- Stoesser,G., Baker,W., van den Broek,A., Camon,E., Garcia-Pastor,M., Kanz,C., Kulikova,T., Leinonen,R., Lin,Q., Lombard,V. et al. (2002) The EMBL nucleotide sequence database. Nucleic Acids Res., 30, 21–26.
[Abstract/Free Full Text] - Benson,D.A., Karsch-Mizrachi,I., Lipman,D.J., Ostell,J., Rapp,B.A. and Wheeler,D.L. (2002) GenBank. Nucleic Acids Res., 30, 17–20.
[Abstract/Free Full Text] - Maidak,B.L., Cole,J.R., Lilburn,T.G., Parker,C.T.,Jr, Saxman,P.R., Farris,R.J., Garrity,G.M., Olsen,G.J., Schmidt,T.M. and Tiedje,J.M. (2001) The RDP-II (Ribosomal Database Project) Nucleic Acids Res., 29, 173–174.
[Abstract/Free Full Text] - Wuyts,J., Van de Peer,Y., Winkelmans,T. and De Wachter,R. (2002) The European database on small subunit ribosomal RNA. Nucleic Acids Res., 30, 183–185.
[Abstract/Free Full Text] - Wuyts,J., De Rijk,P., Van de Peer,Y., Winkelmans,T. and De Wachter,R. (2001) The European Large Subunit Ribosomal RNA Database. Nucleic Acids Res., 29, 175–177.
[Abstract/Free Full Text] - Felsenstein,J., (1989) PHYLIP—Phylogeny Inference Package (version 3.2). Cladistics, 5, 164–166.
- Olsen,G.J., Matsuda,H., Hagstrom,R. and Overbeek,R. (1994) FastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput. Appl. Biosci., 10, 41–48.
[Abstract/Free Full Text] - Adachi,J. and Hasegawa,M. (1996) Molphy Version 2.3, Programs for Molecular Phylogenetics Based on Maximum Likelihood. Technical Report, The Institute of Statistical Mathematics, Tokyo.
- Strimmer,K. and von Haeseler,A. (1996) Quartett Puzzling: a quartett maximum likelihood method for reconstructing tree topologies. Mol. Biol. Evol., 13, 964–969.[Web of Science]
- Stamatakis,A.P., Ludwig,T., Meier,H. and Wolf,M.J. Accelerating parallel maximum likelihood-based phylogenetic tree calculations using subtree equality vectors. Proc. Supercomputing Conference (SC2002), Baltimore, MD, Nov. 2002, IEEE Computer Society.
- Cannone,J.J, Subramanian,S., Schnare,M.N., Collett,J.R., D’Souza,L.M., Du,Y., Feng,B., Lin,N., Madabusi,L.V., Müller,K.M. et al. (2002). The comparative RNA Web (CRW) site: an online database of comparative sequence and structure information for ribosomal, intron and other RNAs. BioMed Central Bioinform., 3, 2.
- Amann,R., Ludwig,W. and Schleifer,K.H. (1995) Phylogentic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev., 59, 143–169.
[Abstract/Free Full Text] - Fuchs,B.M., Wallner,G., Beisker,W., Schwippl,L., Ludwig,W. and Amann,R. (1998) Flow cytrometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl. Environ. Microbiol., 64, 4973–4982.
[Abstract/Free Full Text] - Fuchs,B.M., Syutsubo,K., Ludwig,W. and Amann,R. (2001) In situ accessibility of the Escherichia coli 23S rRNA for fluorescently labeled oligonucleotide probes. Appl. Environ. Microbiol., 67, 961–968.
[Abstract/Free Full Text] - Ludwig,W. and Klenk,H.P. (2001) Overview: a phylogenetic backbone and taxonomic framework for prokaryotic systematics. In Garrity,G. (ed.) Bergey’s Manual of Systematic Bacteriology (2nd edn). Springer, New York, pp. 49–65.
- Kernigham,B.W. and Lin,S. (1970) An efficient heuristic procedure for partitioning graphs. Bell Syst. Tech. J., 49, 291–307.
- Altschul,S.F., Madden,T.L., Schaffer,A.A., Znang,J., Znang,Z., Miller,W. and Lipman,D.J. (1997) Gapped BLAST and PSI-Blast: a new generation of protein-specific gap penalties and weight matrix choice. Nucleic Acids Res., 25, 3389–3402.
[Abstract/Free Full Text] - Pearson,W.R. and Lipman,D.C. (1988) Improved tools for biological sequence comparison. Proc. Natl Acad. Sci. USA, 85, 2444–2448.
[Abstract/Free Full Text] - Thompson,J.D., Higgins,D.G. and Gibson,D.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment. Comput. Appl. Biosci., 8, 189–191.
- Amann,R. and Ludwig,W. (2000) Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology. FEMS Microbiol. Rev., 24, 555–565.[CrossRef][Web of Science][Medline]
- Ludwig,W., Amann,R., Martinez-Romero,E., Schönhuber,W., Bauer,S., Neef,A. and Schleifer,K.H. (1998) rRNA based identification systems for Rhizobia and other bacteria. Plant Soil, 204, 1–9.[CrossRef]
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W. M. Moe, J. Yan, M. F. Nobre, M. S. da Costa, and F. A. Rainey Dehalogenimonas lykanthroporepellens gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2692 - 2697. [Abstract] [Full Text] [PDF]
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K. Mori, K. Yamaguchi, Y. Sakiyama, T. Urabe, and K.-i. Suzuki Caldisericum exile gen. nov., sp. nov., an anaerobic, thermophilic, filamentous bacterium of a novel bacterial phylum, Caldiserica phyl. nov., originally called the candidate phylum OP5, and description of Caldisericaceae fam. nov., Caldisericales ord. nov. and Caldisericia classis nov. Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2894 - 2898. [Abstract] [Full Text] [PDF]
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D. P. Labeda, N. P. Price, R. M. Kroppenstedt, J. M. Donahue, N. M. Williams, and S. F. Sells Streptomyces atriruber sp. nov. and Streptomyces silaceus sp. nov., two novel species of equine origin Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2899 - 2903. [Abstract] [Full Text] [PDF]
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P. Kampfer, N. Lodders, and E. Falsen Luteibacter anthropi sp. nov., isolated from human blood, and reclassification of Dyella yeojuensis Kim et al. 2006 as Luteibacter yeojuensis comb. nov. Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2884 - 2887. [Abstract] [Full Text] [PDF]
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J. Jezbera, A. K. Sharma, U. Brandt, W. F. Doolittle, and M. W. Hahn 'Candidatus Planktophila limnetica', an actinobacterium representing one of the most numerically important taxa in freshwater bacterioplankton Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2864 - 2869. [Abstract] [Full Text] [PDF]
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 K. Abnous, S. P.J. Brooks, J. Kwan, F. Matias, J. Green-Johnson, L. B. Selinger, M. Thomas, and M. Kalmokoff Diets Enriched in Oat Bran or Wheat Bran Temporally and Differentially Alter the Composition of the Fecal Community of Rats J. Nutr., November 1, 2009; 139(11): 2024 - 2031. [Abstract] [Full Text] [PDF]
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M. Ofek, Y. Hadar, and D. Minz Comparison of Effects of Compost Amendment and of Single-Strain Inoculation on Root Bacterial Communities of Young Cucumber Seedlings Appl. Envir. Microbiol., October 15, 2009; 75(20): 6441 - 6450. [Abstract] [Full Text] [PDF]
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M. T. La Duc, S. Osman, P. Vaishampayan, Y. Piceno, G. Andersen, J. A. Spry, and K. Venkateswaran Comprehensive Census of Bacteria in Clean Rooms by Using DNA Microarray and Cloning Methods Appl. Envir. Microbiol., October 15, 2009; 75(20): 6559 - 6567. [Abstract] [Full Text] [PDF]
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 S. A. Amin, D. H. Green, M. C. Hart, F. C. Kupper, W. G. Sunda, and C. J. Carrano Photolysis of iron-siderophore chelates promotes bacterial-algal mutualism PNAS, October 6, 2009; 106(40): 17071 - 17076. [Abstract] [Full Text] [PDF]
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A. V. Vorob'ev, W. de Boer, L. B. Folman, P. L. E. Bodelier, N. V. Doronina, N. E. Suzina, Y. A. Trotsenko, and S. N. Dedysh Methylovirgula ligni gen. nov., sp. nov., an obligately acidophilic, facultatively methylotrophic bacterium with a highly divergent mxaF gene Int J Syst Evol Microbiol, October 1, 2009; 59(10): 2538 - 2545. [Abstract] [Full Text] [PDF]
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E. Stackebrandt, S. Verbarg, A. Fruhling, H.-J. Busse, and B. J. Tindall Dissection of the genus Methylibium: reclassification of Methylibium fulvum as Rhizobacter fulvus comb. nov., Methylibium aquaticum as Piscinibacter aquaticus gen. nov., comb. nov. and Methylibium subsaxonicum as Rivibacter subsaxonicus gen. nov., comb. nov. and emended descriptions of the genera Rhizobacter and Methylibium Int J Syst Evol Microbiol, October 1, 2009; 59(10): 2552 - 2560. [Abstract] [Full Text] [PDF]
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C. Sanchez-Porro, M. A. Amoozegar, R. Rohban, M. Hajighasemi, and A. Ventosa Thalassobacillus cyri sp. nov., a moderately halophilic Gram-positive bacterium from a hypersaline lake Int J Syst Evol Microbiol, October 1, 2009; 59(10): 2565 - 2570. [Abstract] [Full Text] [PDF]
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 C. Hoffmann, D. A. Hill, N. Minkah, T. Kirn, A. Troy, D. Artis, and F. Bushman Community-Wide Response of the Gut Microbiota to Enteropathogenic Citrobacter rodentium Infection Revealed by Deep Sequencing Infect. Immun., October 1, 2009; 77(10): 4668 - 4678. [Abstract] [Full Text] [PDF]
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O. O. Lee, P. Y. Chui, Y. H. Wong, J. R. Pawlik, and P.-Y. Qian Evidence for Vertical Transmission of Bacterial Symbionts from Adult to Embryo in the Caribbean Sponge Svenzea zeai Appl. Envir. Microbiol., October 1, 2009; 75(19): 6147 - 6156. [Abstract] [Full Text] [PDF]
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J.-F. Mangot, C. Lepere, C. Bouvier, D. Debroas, and I. Domaizon Community Structure and Dynamics of Small Eukaryotes Targeted by New Oligonucleotide Probes: New Insight into the Lacustrine Microbial Food Web Appl. Envir. Microbiol., October 1, 2009; 75(19): 6373 - 6381. [Abstract] [Full Text] [PDF]
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L. M. Feazel, L. K. Baumgartner, K. L. Peterson, D. N. Frank, J. K. Harris, and N. R. Pace Opportunistic pathogens enriched in showerhead biofilms PNAS, September 22, 2009; 106(38): 16393 - 16399. [Abstract] [Full Text] [PDF]
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 P. Y. Yung, C. Burke, M. Lewis, S. Egan, S. Kjelleberg, and T. Thomas Phylogenetic screening of a bacterial, metagenomic library using homing endonuclease restriction and marker insertion Nucleic Acids Res., September 18, 2009; (2009) gkp746v1. [Abstract] [Full Text] [PDF]
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D. A. Caron, P. D. Countway, P. Savai, R. J. Gast, A. Schnetzer, S. D. Moorthi, M. R. Dennett, D. M. Moran, and A. C. Jones Defining DNA-Based Operational Taxonomic Units for Microbial-Eukaryote Ecology Appl. Envir. Microbiol., September 15, 2009; 75(18): 5797 - 5808. [Abstract] [Full Text] [PDF]
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R. Lamendella, J. W. Santo Domingo, A. C. Yannarell, S. Ghosh, G. Di Giovanni, R. I. Mackie, and D. B. Oerther Evaluation of Swine-Specific PCR Assays Used for Fecal Source Tracking and Analysis of Molecular Diversity of Swine-Specific "Bacteroidales" Populations Appl. Envir. Microbiol., September 15, 2009; 75(18): 5787 - 5796. [Abstract] [Full Text] [PDF]
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M. Oggerin, D. R. Arahal, V. Rubio, and I. Marin Identification of Beijerinckia fluminensis strains CIP 106281T and UQM 1685T as Rhizobium radiobacter strains, and proposal of Beijerinckia doebereinerae sp. nov. to accommodate Beijerinckia fluminensis LMG 2819 Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2323 - 2328. [Abstract] [Full Text] [PDF]
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P. Anil Kumar, T. N. R. Srinivas, V. Thiel, M. Tank, Ch. Sasikala, Ch. V. Ramana, and J. F. Imhoff Thiohalocapsa marina sp. nov., from an Indian marine aquaculture pond Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2333 - 2338. [Abstract] [Full Text] [PDF]
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M. A. Amoozegar, C. Sanchez-Porro, R. Rohban, M. Hajighasemi, and A. Ventosa Bacillus persepolensis sp. nov., a moderately halophilic bacterium from a hypersaline lake Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2352 - 2358. [Abstract] [Full Text] [PDF]
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P. Kampfer, N. Lodders, M. Vaneechoutte, and G. Wauters Transfer of Sejongia antarctica, Sejongia jeonii and Sejongia marina to the genus Chryseobacterium as Chryseobacterium antarcticum comb. nov., Chryseobacterium jeonii comb. nov. and Chryseobacterium marinum comb. nov. Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2238 - 2240. [Abstract] [Full Text] [PDF]
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S. E. Belova, T. A. Pankratov, E. N. Detkova, E. N. Kaparullina, and S. N. Dedysh Acidisoma tundrae gen. nov., sp. nov. and Acidisoma sibiricum sp. nov., two acidophilic, psychrotolerant members of the Alphaproteobacteria from acidic northern wetlands Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2283 - 2290. [Abstract] [Full Text] [PDF]
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 J. W. Brown, A. Birmingham, P. E. Griffiths, F. Jossinet, R. Kachouri-Lafond, R. Knight, B. F. Lang, N. Leontis, G. Steger, J. Stombaugh, et al. The RNA structure alignment ontology RNA, September 1, 2009; 15(9): 1623 - 1631. [Abstract] [Full Text] [PDF]
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M. Severgnini, P. Cremonesi, C. Consolandi, G. Caredda, G. De Bellis, and B. Castiglioni ORMA: a tool for identification of species-specific variations in 16S rRNA gene and oligonucleotides design Nucleic Acids Res., September 1, 2009; 37(16): e109 - e109. [Abstract] [Full Text] [PDF]
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B. Nehme, Y. Gilbert, V. Letourneau, R. J. Forster, M. Veillette, R. Villemur, and C. Duchaine Culture-Independent Characterization of Archaeal Biodiversity in Swine Confinement Building Bioaerosols Appl. Envir. Microbiol., September 1, 2009; 75(17): 5445 - 5450. [Abstract] [Full Text] [PDF]
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C. Valiente Moro, O. Crouzet, S. Rasconi, A. Thouvenot, G. Coffe, I. Batisson, and J. Bohatier New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of Chlorophyceae and Bacillariophyceae in Environmental Samples Appl. Envir. Microbiol., September 1, 2009; 75(17): 5729 - 5733. [Abstract] [Full Text] [PDF]
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E. Rettedal, S. Vilain, S. Lindblom, K. Lehnert, C. Scofield, S. George, S. Clay, R. S. Kaushik, A. J. M. Rosa, D. Francis, et al. Alteration of the Ileal Microbiota of Weanling Piglets by the Growth-Promoting Antibiotic Chlortetracycline Appl. Envir. Microbiol., September 1, 2009; 75(17): 5489 - 5495. [Abstract] [Full Text] [PDF]
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F. Li, M. A. J. Hullar, Y. Schwarz, and J. W. Lampe Human Gut Bacterial Communities Are Altered by Addition of Cruciferous Vegetables to a Controlled Fruit- and Vegetable-Free Diet J. Nutr., September 1, 2009; 139(9): 1685 - 1691. [Abstract] [Full Text] [PDF]
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N. Youssef, C. S. Sheik, L. R. Krumholz, F. Z. Najar, B. A. Roe, and M. S. Elshahed Comparison of Species Richness Estimates Obtained Using Nearly Complete Fragments and Simulated Pyrosequencing-Generated Fragments in 16S rRNA Gene-Based Environmental Surveys Appl. Envir. Microbiol., August 15, 2009; 75(16): 5227 - 5236. [Abstract] [Full Text] [PDF]
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S. D. Perkins, J. Mayfield, V. Fraser, and L. T. Angenent Potentially Pathogenic Bacteria in Shower Water and Air of a Stem Cell Transplant Unit Appl. Envir. Microbiol., August 15, 2009; 75(16): 5363 - 5372. [Abstract] [Full Text] [PDF]
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E. Lang and E. Stackebrandt Emended descriptions of the genera Myxococcus and Corallococcus, typification of the species Myxococcus stipitatus and Myxococcus macrosporus and a proposal that they be represented by neotype strains. Request for an Opinion Int J Syst Evol Microbiol, August 1, 2009; 59(8): 2122 - 2128. [Abstract] [Full Text] [PDF]
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M. Labrenz, P. A. Lawson, B. J. Tindall, and P. Hirsch Roseibaca ekhonensis gen. nov., sp. nov., an alkalitolerant and aerobic bacteriochlorophyll a-producing alphaproteobacterium from hypersaline Ekho Lake Int J Syst Evol Microbiol, August 1, 2009; 59(8): 1935 - 1940. [Abstract] [Full Text] [PDF]
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A. F. Yassin and H.-J. Busse Psychrobacter lutiphocae sp. nov., isolated from the faeces of a seal Int J Syst Evol Microbiol, August 1, 2009; 59(8): 2049 - 2053. [Abstract] [Full Text] [PDF]
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T. Miyatake, B. J. MacGregor, and H. T. S. Boschker Linking Microbial Community Function to Phylogeny of Sulfate-Reducing Deltaproteobacteria in Marine Sediments by Combining Stable Isotope Probing with Magnetic-Bead Capture Hybridization of 16S rRNA Appl. Envir. Microbiol., August 1, 2009; 75(15): 4927 - 4935. [Abstract] [Full Text] [PDF]
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M. T. Cottrell and D. L. Kirchman Photoheterotrophic Microbes in the Arctic Ocean in Summer and Winter Appl. Envir. Microbiol., August 1, 2009; 75(15): 4958 - 4966. [Abstract] [Full Text] [PDF]
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J. E. McDonald, A. B. de Menezes, H. E. Allison, and A. J. McCarthy Molecular Biological Detection and Quantification of Novel Fibrobacter Populations in Freshwater Lakes Appl. Envir. Microbiol., August 1, 2009; 75(15): 5148 - 5152. [Abstract] [Full Text] [PDF]
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A. Chaudhary, S. K. Haack, J. W. Duris, and T. L. Marsh Bacterial and Archaeal Phylogenetic Diversity of a Cold Sulfur-Rich Spring on the Shoreline of Lake Erie, Michigan Appl. Envir. Microbiol., August 1, 2009; 75(15): 5025 - 5036. [Abstract] [Full Text] [PDF]
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N. Tas, M. H. A. van Eekert, G. Schraa, J. Zhou, W. M. de Vos, and H. Smidt Tracking Functional Guilds: "Dehalococcoides" spp. in European River Basins Contaminated with Hexachlorobenzene Appl. Envir. Microbiol., July 15, 2009; 75(14): 4696 - 4704. [Abstract] [Full Text] [PDF]
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J. Rui, J. Peng, and Y. Lu Succession of Bacterial Populations during Plant Residue Decomposition in Rice Field Soil Appl. Envir. Microbiol., July 15, 2009; 75(14): 4879 - 4886. [Abstract] [Full Text] [PDF]
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R. M. Valster, B. A. Wullings, G. Bakker, H. Smidt, and D. van der Kooij Free-Living Protozoa in Two Unchlorinated Drinking Water Supplies, Identified by Phylogenic Analysis of 18S rRNA Gene Sequences Appl. Envir. Microbiol., July 15, 2009; 75(14): 4736 - 4746. [Abstract] [Full Text] [PDF]
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 P. Cremonesi, G. Pisoni, M. Severgnini, C. Consolandi, P. Moroni, M. Raschetti, and B. Castiglioni Pathogen detection in milk samples by ligation detection reaction-mediated universal array method J Dairy Sci, July 1, 2009; 92(7): 3027 - 3039. [Abstract] [Full Text] [PDF]
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J. P. Davis, N. H. Youssef, and M. S. Elshahed Assessment of the Diversity, Abundance, and Ecological Distribution of Members of Candidate Division SR1 Reveals a High Level of Phylogenetic Diversity but Limited Morphotypic Diversity Appl. Envir. Microbiol., June 15, 2009; 75(12): 4139 - 4148. [Abstract] [Full Text] [PDF]
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 B. Stres, J. M. Tiedje, and B. Murovec BEsTRF: a tool for optimal resolution of terminal-restriction fragment length polymorphism analysis based on user-defined primer-enzyme-sequence databases Bioinformatics, June 15, 2009; 25(12): 1556 - 1558. [Abstract] [Full Text] [PDF]
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S. Borin, L. Brusetti, F. Mapelli, G. D'Auria, T. Brusa, M. Marzorati, A. Rizzi, M. Yakimov, D. Marty, G. J. De Lange, et al. Sulfur cycling and methanogenesis primarily drive microbial colonization of the highly sulfidic Urania deep hypersaline basin PNAS, June 9, 2009; 106(23): 9151 - 9156. [Abstract] [Full Text] [PDF]
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S. M. Pimentel-Elardo, M. Scheuermayer, S. Kozytska, and U. Hentschel Streptomyces axinellae sp. nov., isolated from the Mediterranean sponge Axinella polypoides (Porifera) Int J Syst Evol Microbiol, June 1, 2009; 59(6): 1433 - 1437. [Abstract] [Full Text] [PDF]
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F. D. Andreote, W. L. de Araujo, J. L. de Azevedo, J. D. van Elsas, U. N. da Rocha, and L. S. van Overbeek Endophytic Colonization of Potato (Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities Appl. Envir. Microbiol., June 1, 2009; 75(11): 3396 - 3406. [Abstract] [Full Text] [PDF]
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M. Stieglmeier, R. Wirth, G. Kminek, and C. Moissl-Eichinger Cultivation of Anaerobic and Facultatively Anaerobic Bacteria from Spacecraft-Associated Clean Rooms Appl. Envir. Microbiol., June 1, 2009; 75(11): 3484 - 3491. [Abstract] [Full Text] [PDF]
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P. F. Andeer, D. A. Stahl, N. C. Bruce, and S. E. Strand Lateral Transfer of Genes for Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (RDX) Degradation Appl. Envir. Microbiol., May 15, 2009; 75(10): 3258 - 3262. [Abstract] [Full Text] [PDF]
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M. Herrmann, A. M. Saunders, and A. Schramm Effect of Lake Trophic Status and Rooted Macrophytes on Community Composition and Abundance of Ammonia-Oxidizing Prokaryotes in Freshwater Sediments Appl. Envir. Microbiol., May 15, 2009; 75(10): 3127 - 3136. [Abstract] [Full Text] [PDF]
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H. Cadillo-Quiroz, J. B. Yavitt, and S. H. Zinder Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland Int J Syst Evol Microbiol, May 1, 2009; 59(5): 928 - 935. [Abstract] [Full Text] [PDF]
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E. Jumas-Bilak, L. Roudiere, and H. Marchandin Description of 'Synergistetes' phyl. nov. and emended description of the phylum 'Deferribacteres' and of the family Syntrophomonadaceae, phylum 'Firmicutes' Int J Syst Evol Microbiol, May 1, 2009; 59(5): 1028 - 1035. [Abstract] [Full Text] [PDF]
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D. B. Johnson, P. Bacelar-Nicolau, N. Okibe, A. Thomas, and K. B. Hallberg Ferrimicrobium acidiphilum gen. nov., sp. nov. and Ferrithrix thermotolerans gen. nov., sp. nov.: heterotrophic, iron-oxidizing, extremely acidophilic actinobacteria Int J Syst Evol Microbiol, May 1, 2009; 59(5): 1082 - 1089. [Abstract] [Full Text] [PDF]
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A. F. Yassin Saccharopolyspora rosea sp. nov., isolated from a patient with bronchial carcinoma Int J Syst Evol Microbiol, May 1, 2009; 59(5): 1148 - 1152. [Abstract] [Full Text] [PDF]
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D. H. Choi, G. I. Zhang, J. H. Noh, W.-S. Kim, and B. C. Cho Gracilimonas tropica gen. nov., sp. nov., isolated from a Synechococcus culture Int J Syst Evol Microbiol, May 1, 2009; 59(5): 1167 - 1172. [Abstract] [Full Text] [PDF]
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C. Sanchez-Porro, V. Gallego, H.-J. Busse, P. Kampfer, and A. Ventosa Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., respectively, and emended description of the genus Roseomonas Int J Syst Evol Microbiol, May 1, 2009; 59(5): 1193 - 1198. [Abstract] [Full Text] [PDF]
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S. E. Morales and W. E. Holben Empirical Testing of 16S rRNA Gene PCR Primer Pairs Reveals Variance in Target Specificity and Efficacy Not Suggested by In Silico Analysis Appl. Envir. Microbiol., May 1, 2009; 75(9): 2677 - 2683. [Abstract] [Full Text] [PDF]
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O. Geissinger, D. P. R. Herlemann, E. Morschel, U. G. Maier, and A. Brune The Ultramicrobacterium "Elusimicrobium minutum" gen. nov., sp. nov., the First Cultivated Representative of the Termite Group 1 Phylum Appl. Envir. Microbiol., May 1, 2009; 75(9): 2831 - 2840. [Abstract] [Full Text] [PDF]
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D. P. R. Herlemann, O. Geissinger, W. Ikeda-Ohtsubo, V. Kunin, H. Sun, A. Lapidus, P. Hugenholtz, and A. Brune Genomic Analysis of "Elusimicrobium minutum," the First Cultivated Representative of the Phylum "Elusimicrobia" (Formerly Termite Group 1) Appl. Envir. Microbiol., May 1, 2009; 75(9): 2841 - 2849. [Abstract] [Full Text] [PDF]
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 J. F. Petrosino, S. Highlander, R. A. Luna, R. A. Gibbs, and J. Versalovic Metagenomic Pyrosequencing and Microbial Identification Clin. Chem., May 1, 2009; 55(5): 856 - 866. [Abstract] [Full Text] [PDF]
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R. L. Hansman, S. Griffin, J. T. Watson, E. R. M. Druffel, A. E. Ingalls, A. Pearson, and L. I. Aluwihare The radiocarbon signature of microorganisms in the mesopelagic ocean PNAS, April 21, 2009; 106(16): 6513 - 6518. [Abstract] [Full Text] [PDF]
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P. N. Evans, L. A. Hinds, L. I. Sly, C. S. McSweeney, M. Morrison, and A.-D. G. Wright Community Composition and Density of Methanogens in the Foregut of the Tammar Wallaby (Macropus eugenii) Appl. Envir. Microbiol., April 15, 2009; 75(8): 2598 - 2602. [Abstract] [Full Text] [PDF]
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M. Goberna, H. Insam, and I. H. Franke-Whittle Effect of Biowaste Sludge Maturation on the Diversity of Thermophilic Bacteria and Archaea in an Anaerobic Reactor Appl. Envir. Microbiol., April 15, 2009; 75(8): 2566 - 2572. [Abstract] [Full Text] [PDF]
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L. Speirs, T. Nittami, S. McIlroy, S. Schroeder, and R. J. Seviour Filamentous Bacterium Eikelboom Type 0092 in Activated Sludge Plants in Australia Is a Member of the Phylum Chloroflexi Appl. Envir. Microbiol., April 15, 2009; 75(8): 2446 - 2452. [Abstract] [Full Text] [PDF]
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R. Sekar, L. T. Kaczmarsky, and L. L. Richardson Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals Appl. Envir. Microbiol., April 15, 2009; 75(8): 2581 - 2584. [Abstract] [Full Text] [PDF]
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K. W. Finster, R. A. Herbert, K. U. Kjeldsen, P. Schumann, and B. A. Lomstein Demequina lutea sp. nov., isolated from a high Arctic permafrost soil Int J Syst Evol Microbiol, April 1, 2009; 59(4): 649 - 653. [Abstract] [Full Text] [PDF]
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V. M. Gorlenko, I. A. Bryantseva, S. Rabold, T. P. Tourova, D. Rubtsova, E. Smirnova, V. Thiel, and J. F. Imhoff Ectothiorhodospira variabilis sp. nov., an alkaliphilic and halophilic purple sulfur bacterium from soda lakes Int J Syst Evol Microbiol, April 1, 2009; 59(4): 658 - 664. [Abstract] [Full Text] [PDF]
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S.-Y. Lin, C. C. Young, H. Hupfer, C. Siering, A. B. Arun, W.-M. Chen, W.-A. Lai, F.-T. Shen, P. D. Rekha, and A. F. Yassin Azospirillum picis sp. nov., isolated from discarded tar Int J Syst Evol Microbiol, April 1, 2009; 59(4): 761 - 765. [Abstract] [Full Text] [PDF]
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H. Imachi, S. Sakai, H. Nagai, T. Yamaguchi, and K. Takai Methanofollis ethanolicus sp. nov., an ethanol-utilizing methanogen isolated from a lotus field Int J Syst Evol Microbiol, April 1, 2009; 59(4): 800 - 805. [Abstract] [Full Text] [PDF]
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K. W. Finster, R. A. Herbert, and B. A. Lomstein Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma Int J Syst Evol Microbiol, April 1, 2009; 59(4): 839 - 844. [Abstract] [Full Text] [PDF]
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C. E. Robertson, J. R. Spear, J. K. Harris, and N. R. Pace Diversity and Stratification of Archaea in a Hypersaline Microbial Mat Appl. Envir. Microbiol., April 1, 2009; 75(7): 1801 - 1810. [Abstract] [Full Text] [PDF]
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 T. K. Kim, S. M. Thomas, M. Ho, S. Sharma, C. I. Reich, J. A. Frank, K. M. Yeater, D. R. Biggs, N. Nakamura, R. Stumpf, et al. Heterogeneity of Vaginal Microbial Communities within Individuals J. Clin. Microbiol., April 1, 2009; 47(4): 1181 - 1189. [Abstract] [Full Text] [PDF]
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B. J. Baker, G. W. Tyson, L. Goosherst, and J. F. Banfield Insights into the Diversity of Eukaryotes in Acid Mine Drainage Biofilm Communities Appl. Envir. Microbiol., April 1, 2009; 75(7): 2192 - 2199. [Abstract] [Full Text] [PDF]
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J. S. Blum, S. Han, B. Lanoil, C. Saltikov, B. Witte, F. R. Tabita, S. Langley, T. J. Beveridge, L. Jahnke, and R. S. Oremland Ecophysiology of "Halarsenatibacter silvermanii" Strain SLAS-1T, gen. nov., sp. nov., a Facultative Chemoautotrophic Arsenate Respirer from Salt-Saturated Searles Lake, California Appl. Envir. Microbiol., April 1, 2009; 75(7): 1950 - 1960. [Abstract] [Full Text] [PDF]
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B. K. Harrison, H. Zhang, W. Berelson, and V. J. Orphan Variations in Archaeal and Bacterial Diversity Associated with the Sulfate-Methane Transition Zone in Continental Margin Sediments (Santa Barbara Basin, California) Appl. Envir. Microbiol., March 15, 2009; 75(6): 1487 - 1499. [Abstract] [Full Text] [PDF]
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M. Ast, A. Gruber, S. Schmitz-Esser, H. E. Neuhaus, P. G. Kroth, M. Horn, and I. Haferkamp Diatom plastids depend on nucleotide import from the cytosol PNAS, March 3, 2009; 106(9): 3621 - 3626. [Abstract] [Full Text] [PDF]
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A. F. Yassin, H. Hupfer, H.-P. Klenk, and C. Siering Desmospora activa gen. nov., sp. nov., a thermoactinomycete isolated from sputum of a patient with suspected pulmonary tuberculosis, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 Int J Syst Evol Microbiol, March 1, 2009; 59(3): 454 - 459. [Abstract] [Full Text] [PDF]
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T. Katayama, T. Kato, M. Tanaka, T. A. Douglas, A. Brouchkov, M. Fukuda, F. Tomita, and K. Asano Glaciibacter superstes gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from a permafrost ice wedge Int J Syst Evol Microbiol, March 1, 2009; 59(3): 482 - 486. [Abstract] [Full Text] [PDF]
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M. Arenas, P. I. Banon, J. L. Copa-Patino, C. Sanchez-Porro, A. Ventosa, and J. Soliveri Halomonas ilicicola sp. nov., a moderately halophilic bacterium isolated from a saltern Int J Syst Evol Microbiol, March 1, 2009; 59(3): 578 - 582. [Abstract] [Full Text] [PDF]
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C. Kellermann and C. Griebler Thiobacillus thiophilus sp. nov., a chemolithoautotrophic, thiosulfate-oxidizing bacterium isolated from contaminated aquifer sediments Int J Syst Evol Microbiol, March 1, 2009; 59(3): 583 - 588. [Abstract] [Full Text] [PDF]
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 D. Persoh, M. Melcher, K. Graf, J. Fournier, M. Stadler, and G. Rambold Molecular and morphological evidence for the delimitation of Xylaria hypoxylon Mycologia, March 1, 2009; 101(2): 256 - 268. [Abstract] [Full Text] [PDF]
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B. H. G. W. van Houten, R. J. W. Meulepas, W. van Doesburg, H. Smidt, G. Muyzer, and A. J. M. Stams Desulfovibrio paquesii sp. nov., a hydrogenotrophic sulfate-reducing bacterium isolated from a synthesis-gas-fed bioreactor treating zinc- and sulfate-rich wastewater Int J Syst Evol Microbiol, February 1, 2009; 59(2): 229 - 233. [Abstract] [Full Text] [PDF]
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S. P. Glaeser, P. Kampfer, H.-J. Busse, S. Langer, and J. Glaeser Novosphingobium acidiphilum sp. nov., an acidophilic salt-sensitive bacterium isolated from the humic acid-rich Lake Grosse Fuchskuhle Int J Syst Evol Microbiol, February 1, 2009; 59(2): 323 - 330. [Abstract] [Full Text] [PDF]
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J. Wiese, V. Thiel, A. Gartner, R. Schmaljohann, and J. F. Imhoff Kiloniella laminariae gen. nov., sp. nov., an alphaproteobacterium from the marine macroalga Laminaria saccharina Int J Syst Evol Microbiol, February 1, 2009; 59(2): 350 - 356. [Abstract] [Full Text] [PDF]
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I. S. Kulichevskaya, O. I. Baulina, P. L. E. Bodelier, W. I. C. Rijpstra, J. S. S. Damste, and S. N. Dedysh Zavarzinella formosa gen. nov., sp. nov., a novel stalked, Gemmata-like planctomycete from a Siberian peat bog Int J Syst Evol Microbiol, February 1, 2009; 59(2): 357 - 364. [Abstract] [Full Text] [PDF]
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J. Pinhassi, M. J. Pujalte, J. Pascual, J. M. Gonzalez, I. Lekunberri, C. Pedros-Alio, and D. R. Arahal Bermanella marisrubri gen. nov., sp. nov., a genome-sequenced gammaproteobacterium from the Red Sea Int J Syst Evol Microbiol, February 1, 2009; 59(2): 373 - 377. [Abstract] [Full Text] [PDF]
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C. Sanchez-Porro, R. R. de la Haba, N. Soto-Ramirez, M. C. Marquez, R. Montalvo-Rodriguez, and A. Ventosa Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov. Int J Syst Evol Microbiol, February 1, 2009; 59(2): 397 - 405. [Abstract] [Full Text] [PDF]
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