Nucleic Acids Research 2005 33(6):2060; doi:10.1093/nar/gki340
Published online 7 April 2005
© The Author 2005. Published by Oxford University Press. All rights reserved
Corrigendum |
Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR
The authors would like to apologize for failing to correct an error in their description of the two-step PCR used in their protocol, which reverses the order of the primer pairs used for the first and second rounds of PCR. This error is found in two locations in the manuscript:
On page 2, ‘Two rounds of amplification were used, an initial round of 35 cycles with the inner primer set, followed by a second round of 35 cycles with the corresponding outer primer set.’ should be changed to ‘Two rounds of amplification were used, an initial round of 35 cycles with the outer primer set, followed by a second round of 35 cycles with the corresponding inner primer set.’
On page 3, in the legend for Figure 1, ‘Second round PCRs (outer primers) were performed with a dilution of the first round (inner primers) reaction.’ should be changed to ‘Second round PCRs (inner primers) were performed with a dilution of the first round (outer primers) reaction.’
The correct order of primer sets that they used for two-step PCR is outer primer set for the first round, followed by inner primer set for the second round.
This descriptive error does not alter any of the conclusions of the paper. The authors regret any inconvenience or misunderstanding that their error may have caused.
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