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PHYML Online—a web server for fast maximum likelihood-based phylogenetic inference
Bioinformatics Institute and Allan Wilson Centre, University of Auckland Private Bag 92019, Auckland, New Zealand 1Projet Méthodes et Algorithmes pour la Bioinformatique, LIRMM-CNRS 161 Rue Ada, 34392-Montpellier Cedex 5, France
*To whom correspondence should be addressed. Tel: +33 4 67 41 85 47; Fax: +33 4 67 41 85 00; Email: gascuel{at}lirmm.fr
Received December 8, 2004. Accepted December 20, 2004.
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONALGORITHMPHYML ONLINEAVAILABILITYREFERENCES |
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PHYML Online is a web interface to PHYML, a software that implements a fast and accurate heuristic for estimating maximum likelihood phylogenies from DNA and protein sequences. This tool provides the user with a number of options, e.g. nonparametric bootstrap and estimation of various evolutionary parameters, in order to perform comprehensive phylogenetic analyses on large datasets in reasonable computing time. The server and its documentation are available at http://atgc.lirmm.fr/phyml.
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONALGORITHMPHYML ONLINEAVAILABILITYREFERENCES |
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The ever-increasing size of homologous sequence datasets and complexity of substitution models stimulate the development of better methods for building phylogenetic trees. Likelihood-based approaches (including Bayesian) provided arguably the most successful advances in this area in the last decade. Unfortunately, these methods are hampered with computational difficulties. Different strategies have then been used to tackle this problem, mostly based on stochastic approaches. Markov chain Monte Carlo methods are probably the most valuable tools in this context as they provide computationally tractable solutions to Bayesian estimation of phylogenies (1,2).
Stochastic approaches have also been used to address optimization issues in the maximum likelihood framework. Hence, simulated annealing (3) and genetic algorithms (4,5) were proposed to estimate maximum likelihood phylogenies from large datasets. However, the hill climbing principle is usually considered faster than stochastic optimization and sufficient for numerous combinatorial optimization problems (6). Recently, Guindon and Gascuel (7) described a fast and simple heuristic based on this principle, for building maximum likelihood phylogenies. Several simulation studies (7,8) demonstrated that the tree topologies estimated with this approach are as accurate as those inferred using the best tree building methods currently available. These studies also showed that this new method is considerably faster than the other likelihood-based approaches. Using this heuristic, the analysis of large datasets is now achieved in reasonable computing time on any standard personal computer; e.g. only 12 min were required to analyse a dataset consisting of 500 rbcL sequences with 1428 bp from plant plastids.
This paper introduces PHYML Online, a web interface to the PHYML (PHYlogenetic inferences using Maximum Likelihood) software that implements the heuristic described by Guindon and Gascuel (7). PHYML Online provides a number of useful options (e.g. nonparametric bootstrap), and proposes quite recent models of sequence evolution [e.g. WAG (9) and DCMut (10)]. We first give an overview of the algorithm and present the web server thereafter.
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ALGORITHM |
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TOPABSTRACTINTRODUCTIONALGORITHMPHYML ONLINEAVAILABILITYREFERENCES |
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The core of the heuristic is based on a well-known tree-swapping operation, namely ‘nearest neighbour interchange’, which defines three possible topological configurations around each internal branch (11). For each of these configurations, the length of the internal branch that maximizes the likelihood is estimated using numerical optimization. The difference of likelihood obtained under the best alternative topological configuration and the current one defines a score. A score with positive value indicates that the best alternative topological configuration yields an improvement of likelihood. A score with negative value indicates that the current topological configuration cannot be improved at this stage and only the length of the internal branch is adjusted. Each internal branch is examined in this manner and ranked according to its score. The optimal length of external branches is also computed. These calculations are performed independently for every branch and they define a set of (topological or numerical) modifications, each of which corresponds to an improvement of the current tree regarding the likelihood function.
The standard approach would only apply one of these modifications, typically that corresponding to the internal branch with best score. Here, a large proportion of all modifications computed previously is performed instead. This proportion is adjusted so as to increase the likelihood at each step, ensuring convergence of the algorithm. This way, the current tree is improved at each step, both in terms of topology and branch length, and only a few steps (usually a few dozen or less) are necessary to reach an optimum of the likelihood function. This explains the speed of this algorithm whose time complexity is O(pns), where p represents the number of refinement steps that have been performed and n is the number of sequences of length s.
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PHYML ONLINE |
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TOPABSTRACTINTRODUCTIONALGORITHMPHYML ONLINEAVAILABILITYREFERENCES |
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PHYML Online is a web interface to the PHYML algorithm (Figure 1). By default, the input data consists of a single text file containing one or more alignments of DNA or protein sequences in PHYLIP (12) interleaved or sequential format. Examples of sequence datasets in PHYLIP format are given in the ‘User's guide’ section of the web site.
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Setting the parameters of a phylogenetic analysis through the interface is straightforward. The first step is the selection of the substitution model of interest. Alignments of homologous DNA and amino acid sequences can be examined under a wide range of models (JC69, K80, F81, F84, HKY85, TN93 and GTR for nucleotides, and Dayhoff, JTT, mtREV, WAG and DCMut for amino acids). Variability of substitution rates across sites and invariable sites can also be taken into account. The parameters that model the intensity of the variation of rates across sites and the proportion of invariables sites can be fixed by the user or estimated by maximum likelihood. Note that the parameters of the substitution model can be estimated under a fixed tree topology or not. The fixed topology option is useful when describing the evolutionary process is more important than estimating the history of sequences.
An option is available to assess the reliability of internal branches using nonparametric bootstrap (13) which is possible to achieve for even large datasets, thanks to the speed of PHYML optimization algorithm. The number of bootstrap replicates is fixed by the user. The bootstrap values are displayed on the maximum likelihood phylogeny estimated from the original dataset. Trees estimated from each bootstrap replicate, as well as the corresponding substitution parameters, can also be saved in separate files for further analysis (e.g. computation of confidence intervals for the substitution parameters or estimation of a consensus bootstrap tree, as performed by PHYLIP's CONSENSE).
Several datasets can be analysed in a single run. This option is especially useful in multiple gene studies. Multiple trees can also be used as input and further optimized by the algorithm described above. This might prevent the tree searching heuristic to be trapped in local maxima. When combined with the fixed tree option, the multiple input trees approach also facilitates the comparison of the fit of different phylogenies estimated from a single dataset. The ‘User's guide’ section gives details on the format of multiple sequence and tree files.
Sequences [and starting tree(s) if provided] are uploaded on our server, a 16-processor IBM computer running Linux 2.6.8-1.521custom SMP, and a maximum likelihood analysis is performed using the PHYML algorithm. Results are then sent to the user by electronic mail. The first file presents a summary of the options selected by the user, maximum likelihood estimates of the parameters of the substitution model that were adjusted, and the log likelihood of the model given the data. The second file shows the maximum likelihood phylogeny(ies) in NEWICK format. Trees can be viewed through an applet available on the PHYML Online server. This applet runs the program ATV (14) that provides numerous options to display and manipulate large phylogenetic trees.
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AVAILABILITY |
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TOPABSTRACTINTRODUCTIONALGORITHMPHYML ONLINEAVAILABILITYREFERENCES |
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The PHYML Online server is located at ‘Laboratoire d'Informatique, de Robotique et de Microélectronique de Montpellier’: http://atgc.lirmm.fr/phyml.
PHYML can also be downloaded for local installation at http://atgc.lirmm.fr/phyml/binaries.html. The PHYML software has been implemented in C ANSI and is available under GNU general public licence. Sources are available upon request. Binaries, example datasets, sources and documentation are distributed free of charge for academic purpose only.
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ACKNOWLEDGEMENTS |
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Thanks to Emmanuel Douzery and Stephanie Plön for carefully reading this article. This work was funded by ACI IMPBIO (French Ministry of Research) and Réseau des Génopoles. S.G. is supported by a postdoctoral fellowship from the Allan Wilson Centre for Molecular Ecology and Evolution, New Zealand. Funding to pay the Open Access publication charges for this article was provided by CNRS-STIC.
Conflict of interest statement. None declared.
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REFERENCES |
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TOPABSTRACTINTRODUCTIONALGORITHMPHYML ONLINEAVAILABILITYREFERENCES |
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- Rannala, B. and Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference J. Mol. Evol., 43, 304–311[Web of Science][Medline] .
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[Abstract/Free Full Text] . - Salter, L. and Pearl, D. (2001) Stochastic search strategy for estimation of maximum likelihood phylogenetic trees Syst. Biol., 50, 7–17
[Abstract/Free Full Text] . - Lewis, P. (1998) A genetic algorithm for maximum likelihood phylogeny inference using nucleotide sequence data Mol. Biol. Evol., 15, 277–283[Abstract] .
- Lemmon, A. and Milinkovitch, M. (2002) The metapopulation genetic algorithm: an efficient solution for the problem of large phylogeny estimation Proc. Natl Acad. Sci. USA, 99, 10516–10521
[Abstract/Free Full Text] . - In Aarts, E. and Lenstra, J.K. (Eds.). Local Search in Combinatorial Optimization, (1997) Chichester, UK Wiley .
- Guindon, S. and Gascuel, O. (2003) A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood Syst. Biol., 52, 696–704
[Abstract/Free Full Text] . - Vinh, L.S. and von Haeseler, A. (2004) IQPNNI: moving fast through tree space and stopping in time Mol. Biol. Evol., 21, 1565–1571
[Abstract/Free Full Text] . - Whelan, S. and Goldman, N. (2001) A general empirical model of protein evolution derived from multiple protein families using a maximum-likelihood approach Mol. Biol. Evol., 18, 691–699
[Abstract/Free Full Text] . - Kosiol, C. and Goldman, N. (2005) Different versions of the Dayhoff rate matrix Mol. Biol. Evol., 22, 193–199
[Abstract/Free Full Text] . - Swofford, D., Olsen, G., Waddel, P., Hillis, D. (1996) Phylogenetic inference In Hillis, D., Moritz, C., Mable, B. (Eds.). Molecular Systematics, chapter 11 Sinauer Sunderland, MA .
- Felsenstein, J. (1993) PHYLIP (PHYLogeny Inference Package) version 3.6a2, Distributed by the author, Department of Genetics, University of Washington, Seattle, WA .
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E. Rottinger, A. Saudemont, V. Duboc, L. Besnardeau, D. McClay, and T. Lepage FGF signals guide migration of mesenchymal cells, control skeletal morphogenesis and regulate gastrulation during sea urchin development Development, January 15, 2008; 135(2): 353 - 365. [Abstract] [Full Text] [PDF]
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E. V. Kriventseva, N. Rahman, O. Espinosa, and E. M. Zdobnov OrthoDB: the hierarchical catalog of eukaryotic orthologs Nucleic Acids Res., January 11, 2008; 36(suppl_1): D271 - D275. [Abstract] [Full Text] [PDF]
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A. Gartner, J. Wiese, and J. F. Imhoff Amphritea atlantica gen. nov., sp. nov., a gammaproteobacterium from the Logatchev hydrothermal vent field Int J Syst Evol Microbiol, January 1, 2008; 58(1): 34 - 39. [Abstract] [Full Text] [PDF]
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A. G. Koziol and D. G. Durnford Euglena Light-Harvesting Complexes Are Encoded by Multifarious Polyprotein mRNAs that Evolve in Concert Mol. Biol. Evol., January 1, 2008; 25(1): 92 - 100. [Abstract] [Full Text] [PDF]
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Y. Jiang, S.-K. Tang, J. Wiese, L.-H. Xu, J. F. Imhoff, and C.-L. Jiang Streptomyces hainanensis sp. nov., a novel member of the genus Streptomyces Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2694 - 2698. [Abstract] [Full Text] [PDF]
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D. R. Brown, R. F. Whitcomb, and J. M. Bradbury Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes) Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2703 - 2719. [Abstract] [Full Text] [PDF]
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R. Range, F. Lapraz, M. Quirin, S. Marro, L. Besnardeau, and T. Lepage Cis-regulatory analysis of nodal and maternal control of dorsal-ventral axis formation by Univin, a TGF-{beta} related to Vg1 Development, October 15, 2007; 134(20): 3649 - 3664. [Abstract] [Full Text] [PDF]
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A. K. Knutsen, V. Robert, G. A. Poot, W. Epping, M. Figge, A. Holst-Jensen, I. Skaar, and M. Th. Smith Polyphasic re-examination of Yarrowia lipolytica strains and the description of three novel Candida species: Candida oslonensis sp. nov., Candida alimentaria sp. nov. and Candida hollandica sp. nov. Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2426 - 2435. [Abstract] [Full Text] [PDF]
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D. Leduc, A. Battesti, and E. Bouveret The Hotdog Thioesterase EntH (YbdB) Plays a Role In Vivo in Optimal Enterobactin Biosynthesis by Interacting with the ArCP Domain of EntB J. Bacteriol., October 1, 2007; 189(19): 7112 - 7126. [Abstract] [Full Text] [PDF]
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P. Narasingarao and M. M. Haggblom Pelobacter seleniigenes sp. nov., a selenate-respiring bacterium Int J Syst Evol Microbiol, September 1, 2007; 57(9): 1937 - 1942. [Abstract] [Full Text] [PDF]
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R. R. Copley, M. Totrov, J. Linnell, S. Field, J. Ragoussis, and I. A. Udalova Functional conservation of Rel binding sites in drosophilid genomes Genome Res., September 1, 2007; 17(9): 1327 - 1335. [Abstract] [Full Text] [PDF]
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R. Kostanjsek, J. Strus, and G. Avgustin "Candidatus Bacilloplasma," a Novel Lineage of Mollicutes Associated with the Hindgut Wall of the Terrestrial Isopod Porcellio scaber (Crustacea: Isopoda) Appl. Envir. Microbiol., September 1, 2007; 73(17): 5566 - 5573. [Abstract] [Full Text] [PDF]
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 M. J Agulleiro, M. Andre, S. Morais, J. Cerda, and P. J Babin High Transcript Level of Fatty Acid-Binding Protein 11 but Not of Very Low-Density Lipoprotein Receptor Is Correlated to Ovarian Follicle Atresia in a Teleost Fish (Solea senegalensis) Biol Reprod, September 1, 2007; 77(3): 504 - 516. [Abstract] [Full Text] [PDF]
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S. Kirchberger, M. Leroch, M. A. Huynen, M. Wahl, H. E. Neuhaus, and J. Tjaden Molecular and Biochemical Analysis of the Plastidic ADP-glucose Transporter (ZmBT1) from Zea mays J. Biol. Chem., August 3, 2007; 282(31): 22481 - 22491. [Abstract] [Full Text] [PDF]
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J. Tarraga, I. Medina, L. Arbiza, J. Huerta-Cepas, T. Gabaldon, J. Dopazo, and H. Dopazo Phylemon: a suite of web tools for molecular evolution, phylogenetics and phylogenomics Nucleic Acids Res., July 13, 2007; 35(suppl_2): W38 - W42. [Abstract] [Full Text] [PDF]
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T. Osanai and K. Tanaka Keeping in Touch with PII: PII-Interacting Proteins in Unicellular Cyanobacteria Plant Cell Physiol., July 1, 2007; 48(7): 908 - 914. [Abstract] [Full Text] [PDF]
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A. G. Koziol, T. Borza, K.-I. Ishida, P. Keeling, R. W. Lee, and D. G. Durnford Tracing the Evolution of the Light-Harvesting Antennae in Chlorophyll a/b-Containing Organisms Plant Physiology, April 1, 2007; 143(4): 1802 - 1816. [Abstract] [Full Text] [PDF]
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 T. Sato, H. Atomi, and T. Imanaka Archaeal Type III RuBisCOs Function in a Pathway for AMP Metabolism Science, February 16, 2007; 315(5814): 1003 - 1006. [Abstract] [Full Text] [PDF]
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M. Spencer, D. Bryant, and E. Susko Conditioned Genome Reconstruction: How to Avoid Choosing the Conditioning Genome Syst Biol, February 1, 2007; 56(1): 25 - 43. [Abstract] [Full Text] [PDF]
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T. Nosenko, K. L. Lidie, F. M. Van Dolah, E. Lindquist, J.-F. Cheng, US Department of Energy-Joint Genome Institute, and D. Bhattacharya Chimeric Plastid Proteome in the Florida "Red Tide" Dinoflagellate Karenia brevis Mol. Biol. Evol., November 1, 2006; 23(11): 2026 - 2038. [Abstract] [Full Text] [PDF]
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T. A. Richards, J. B. Dacks, S. A. Campbell, J. L. Blanchard, P. G. Foster, R. McLeod, and C. W. Roberts Evolutionary origins of the eukaryotic shikimate pathway: gene fusions, horizontal gene transfer, and endosymbiotic replacements. Eukaryot. Cell, September 1, 2006; 5(9): 1517 - 1531. [Abstract] [Full Text] [PDF]
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A M Tarrant, S R Greytak, G V Callard, and M E Hahn Estrogen receptor-related receptors in the killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness. J. Mol. Endocrinol., August 1, 2006; 37(1): 105 - 120. [Abstract] [Full Text] [PDF]
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A. Shahsavarani, B. McNeill, F. Galvez, C. M. Wood, G. G. Goss, P.-P. Hwang, and S. F. Perry Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout (Oncorhynchus mykiss) J. Exp. Biol., May 15, 2006; 209(10): 1928 - 1943. [Abstract] [Full Text] [PDF]
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M. E. Schranz and T. Mitchell-Olds Independent Ancient Polyploidy Events in the Sister Families Brassicaceae and Cleomaceae PLANT CELL, May 1, 2006; 18(5): 1152 - 1165. [Abstract] [Full Text] [PDF]
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M. Salomao, X. An, X. Guo, W. B. Gratzer, N. Mohandas, and A. J. Baines Mammalian {alpha}I-spectrin is a neofunctionalized polypeptide adapted to small highly deformable erythrocytes PNAS, January 17, 2006; 103(3): 643 - 648. [Abstract] [Full Text] [PDF]
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P. Kosa, M. Valach, L. Tomaska, K. H. Wolfe, and J. Nosek Complete DNA sequences of the mitochondrial genomes of the pathogenic yeasts Candida orthopsilosis and Candida metapsilosis: insight into the evolution of linear DNA genomes from mitochondrial telomere mutants. Nucleic Acids Res., January 1, 2006; 34(8): 2472 - 2481. [Abstract] [Full Text] [PDF]
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