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Nucleic Acids Research 2006 34(2):417-425; doi:10.1093/nar/gkj446
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Published online 13 January 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
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Article

Pol{eta}, Pol{zeta} and Rev1 together are required for G to T transversion mutations induced by the (+)- and (–)-trans-anti-BPDE-N2-dG DNA adducts in yeast cells

Bo Zhao, Jillian Wang, Nicholas E. Geacintov1 and Zhigang Wang*

Graduate Center for Toxicology, University of Kentucky Lexington, KY 40536, USA 1Department of Chemistry, New York University New York, NY 10003, USA

*To whom correspondence should be addressed. Tel: +1 859 323 5784; Fax: +1 859 323 1059; Email: zwang{at}uky.edu

Received November 11, 2005. Revised January 2, 2006. Accepted January 2, 2006.


   ABSTRACT
TOP
 ABSTRACT
INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES
 
Benzo[a]pyrene is an important environmental mutagen and carcinogen. Its metabolism in cells yields the mutagenic, key ultimate carcinogen 7R,8S,9S,10R-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide, (+)-anti-BPDE, which reacts via its 10-position with N2-dG in DNA to form the adduct (+)-trans-anti-BPDE-N2-dG. To gain molecular insights into BPDE-induced mutagenesis, we examined in vivo translesion synthesis and mutagenesis in yeast cells of a site-specific 10S (+)-trans-anti-BPDE-N2-dG adduct and the stereoisomeric 10R (–)-trans-anti-BPDE-N2-dG adduct. In wild-type cells, bypass products consisted of 76% C, 14% A and 7% G insertions opposite (+)-trans-anti-BPDE-N2-dG; and 89% C, 4% A and 4% G insertions opposite (–)-trans-anti-BPDE-N2-dG. Translesion synthesis was reduced by ~26–37% in rad30 mutant cells lacking Pol{eta}, but more deficient in rev1 and almost totally deficient in rev3 (lacking Pol{zeta}) mutants. C insertion opposite the lesion was reduced by ~24–33% in rad30 mutant cells, further reduced in rev1 mutant, and mostly disappeared in the rev3 mutant strain. The insertion of A was largely abolished in cells lacking either Pol{eta}, Pol{zeta} or Rev1. The insertion of G was not detected in either rev1 or rev3 mutant cells. The rad30 rev3 double mutant exhibited a similar phenotype as the single rev3 mutant with respect to translesion synthesis and mutagenesis. These results show that while the Pol{zeta} pathway is generally required for translesion synthesis and mutagenesis of the (+)- and (–)-trans-anti-BPDE-N2-dG DNA adducts, Pol{eta}, Pol{zeta} and Rev1 together are required for G->T transversion mutations, a major type of mutagenesis induced by these lesions. Based on biochemical and genetic results, we present mechanistic models of translesion synthesis of these two DNA adducts, involving both the one-polymerase one-step and two-polymerase two-step models.


   INTRODUCTION
TOP
 ABSTRACT
 INTRODUCTION
MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES
 
Polycyclic aromatic hydrocarbons (PAH) are a class of common environmental pollutants that are produced by the incomplete combustion of organic materials. Benzo[a]pyrene is a widely studied PAH compound due to its potent carcinogenic activity in animal models. The chemically unreactive benzo[a]pyrene is metabolized in cells, forming reactive diol epoxide derivatives that can bind covalently to DNA. Unrepaired benzo[a]pyrene DNA adducts can lead to mutations that may eventually result in cancer. The reactive and mutagenic metabolites of benzo[a]pyrene are the (+)-7R,8S,9S,10R-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide, (+)-anti-BPDE and the (–)-7R,8S,9S,10R enantiomer, (–)-anti-BPDE (1,2). DNA damage occurs mainly by the binding of the C10 position of anti-BPDE to the N2 position of guanine, thus forming the four stereoisomeric bulky adducts 10S (+)-trans-anti-BPDE-N2-dG, 10R (+)-cis-anti-BPDE-N2-dG, 10R (–)-trans-anti-BPDE-N2-dG and 10S (–)-cis-anti-BPDE-N2-dG (2,3). In vitro, the reaction of (+)-anti-BPDE with DNA yields predominantly the (+)-trans-anti-BPDE-N2-dG adduct, while the reaction of (–)-anti-BPDE generates mainly the (–)-trans-anti-BPDE-N2-dG adduct (4). In cells, the major benzo[a]pyrene DNA adduct is (+)-trans-anti-BPDE-N2-dG (2).

The structures of the four anti-BPDE-N2-dG adducts in DNA have been solved by NMR spectroscopy (5). In a duplex DNA containing a (+)- or (–)-trans-anti-BPDE-N2-dG adduct, the pyrenyl residues are not intercalated between adjacent base pairs. Instead, in the case of the (+)-trans-anti-BPDE-N2-dG adduct, the aromatic pyrenyl residue stacks primarily over an adjacent sugar ring in the complementary strand in the minor groove, and is oriented toward the 5' end of the modified strand. In the case of the (–)-trans-anti-BPDE-N2-dG adduct, the pyrenyl residue stacks mainly over a sugar ring in the complementary strand in the minor groove, and is oriented toward the 3' end of the modified strand (5–7). Thus, despite their identical chemical structure, the stereoisomeric (+)- or (–)-trans-anti-BPDE-N2-dG adducts adopt different configurations in duplex DNA as a result of the different absolute configurations of substituents about the four chiral carbon atoms. This difference, however, does not appear to affect the efficiency of their removal by human nucleotide excision repair in vitro (8).

BPDE DNA adducts are mutagenic in a variety of prokaryotic and eukaryotic cellular systems (9–15). However, the molecular mechanism of BPDE-induced mutagenesis is not well understood in eukaryotes. In cells, base damage-induced mutagenesis is mainly mediated through error-prone translesion synthesis. Key steps of translesion synthesis include copying damaged sites of the template by specialized polymerases during DNA replication. Conceptually, copying the lesion site may be divided into two distinct steps: nucleotide insertion opposite the lesion and extension synthesis from opposite the lesion. Recent studies indicate that Pol{zeta} and the Y family polymerases are important translesion polymerases in eukaryotes [reviewed in Refs (16–20)]. In the yeast Saccharomyces cerevisiae, the Y family consists of Pol{eta} and Rev1 (21). Mammals contain two additional members of the Y family polymerases: Pol{kappa} and Pol{iota} (16,21).

Translesion synthesis can be error-free or error-prone. Whereas error-prone translesion synthesis results in mutagenesis, error-free translesion synthesis suppresses mutagenesis. In vitro, Pol{kappa} effectively performs error-free translesion synthesis in response to (+)- and (–)-trans-anti-BPDE-N2-dG DNA adducts (22–26), whereas human Pol{eta} is capable of error-prone nucleotide insertion opposite these lesions (23,27–29). In vivo, Pol{kappa} indeed plays an important role in suppressing BPDE-induced mutagenesis (30), and is required for recovery from the BPDE-induced S-phase checkpoint (31). In yeast cells, there is evidence supporting a role for Pol{eta} in error-pone translesion synthesis following exposure of DNA to (±)-anti-BPDE (15). The majority of mutations induced by (±)-anti-BPDE in yeast, however, is generated through the Pol{zeta} mutagenesis pathway that includes the Rev1 protein (15). Similarly, mutagenesis following cellular exposure to racemic (±)-anti-BPDE also requires Pol{zeta} and Rev1 in cultured human cells (32,33).

The treatment of plasmid DNA or cells with racemic (±)-anti-BPDE yields several different types of DNA adducts. Therefore, it is difficult to define the roles of Pol{eta}, Pol{zeta} and Rev1 in BPDE-induced mutagenesis with DNA adducts of defined structure based on the treatment of DNA or cells with racemic (±)-anti-BPDE. In order to understand BPDE mutagenesis at a more defined molecular level, we examined in vivo translesion synthesis of two site-specific (+)- and (–)-trans-anti-BPDE-N2-dG adducts in yeast cells. In this report, we show that while the Pol{zeta} pathway is generally required for translesion synthesis and mutagenesis of the (+)- and (–)-trans-anti-BPDE-N2-dG DNA adducts, Pol{eta}, Pol{zeta} and Rev1 together are required for G->T transversion mutations, a major type of mutagenesis induced by these lesions. Based on in vitro biochemical and in vivo genetic results, we present mechanistic models of translesion synthesis and mutagenesis of these two DNA adducts.


   MATERIALS AND METHODS
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
RESULTS
 DISCUSSION
 REFERENCES
 
Materials
T4 DNA ligase, the T4 gene 32 protein and T4 polynucleotide kinase were obtained from Enzymax (Lexington, KY). Yeast lytic enzyme (70 000 U/g) was purchased from MP Biomedicals (Irvine, CA). The Wizard PCR Preps DNA Purification Resin was from Promega (Wisconsin, WI). The Thermo Sequenase kit was obtained from Amersham Pharmacia Biotech (Piscataway, NJ). Oligonucleotides containing a site-specific (+)-trans-anti-N2-dG or a (–)-trans-anti-BPDE-N2-dG adduct was prepared as described previously (34–36). Its sequence is 5'-CTCGATCGCTAACGCTACCATCCGAATTCGCCC-3', where the modified guanine is underlined. Other DNA oligonucleotides were synthesized by Integrated DNA Technologies (Coralville, IA).

Yeast strains
Yeast strains used were the wild-type BY4741 (MATa his3 leu2 met15 ura3) and the isogenic BY4741{Delta}rad30 (rad30 deletion mutant), BY4741{Delta}rev1 (rev1 deletion mutant), BY4741{Delta}rev3 (rev3 deletion mutant) and BY4741{Delta}rev3{Delta}rad30 (rev3 rad30 double deletion mutant). BY4741 was purchased from ATCC (Manassas, VA). BY4741{Delta}rad30 (lacking Pol{eta}) was purchased from Research Genetics (Huntsville, AL). BY4741{Delta}rev1, BY4741{Delta}rev3 (lacking Pol{zeta}) and BY4741{Delta}rev3{Delta}rad30 were constructed as described previously (15,37).

Construction of plasmids containing site-specific (+)-trans-anti-BPDE-N2-dG or (–)-trans-anti-BPDE-N2-dG adducts
Plasmids containing site-specific (+)-trans-anti-BPDE-N2-dG or (–)-trans-anti-BPDE-N2-dG adducts were constructed by Enzymax using a previously described method (37). Briefly, a 20mer DNA oligonucleotide, 5'-GTGCCCTCCATGGAAAAATC-3', was annealed to the single-stranded phagemid pELUf1 vector at its unique NcoI restriction site within the URA3 gene. Following digestion with the NcoI restriction endonuclease, the linearized pELUf1 was annealed with a 62mer DNA scaffold, 5'-CTGUGCCCUCCAUGGGGCGAAUTUGGAUGGUAGCGUTAGCGAUCGAGGAAAAAUCAGTCAAG-3', and the damaged 33mer oligonucleotide that had been phosphorylated at the 5' end by T4 polynucleotide kinase. While the mid region of the scaffold is complementary to the damaged oligonucleotide, its ends are complementary to the single stranded pELUf1 ends. The BPDE-modified oligonucleotide was ligated into the pELUf1 vector by T4 DNA ligase at 16°C for 20 h, and the DNA was precipitated in ethanol. Finally, the complementary strand of pELUf1 was synthesized with T4 DNA polymerase in the presence of T4 gene 32 protein and 0.5 mM each of dATP, dCTP, dGTP and dUTP, using the scaffold as the primer. The resulting construct was a double-stranded plasmid containing a site-specific (+)-trans-anti-BPDE-N2-dG or (–)-trans-anti-BPDE-N2-dG adduct, in which the undamaged strand contained U in place of T. Formation of double-stranded plasmid pELUf1-BPDE was confirmed by electrophoresis on a 1% agarose gel.

In vivo translesion synthesis assays in yeast cells
In vivo translesion synthesis assays were performed according to a previously described method (37) with modifications. Briefly, site-specifically damaged pELUf-BPDE plasmid (2 µg) was transformed into yeast cells of various strains by the lithium acetate method (38). Following transformation, yeast cells were collected by centrifugation (20 s at 5000 r.p.m.) in a microcentrifuge. Cells were resuspended in 400 µl of sterile water and were plated onto two YNB minimal agar (0.17% yeast nitrogen base, 0.49% ammonium sulfate, 2% glucose and 2% agar) plates lacking leucine but supplemented with 5 mM 5-fluoroorotic acid (5-FOA), 150 µM methionine and 380 µM uracil to score for colonies containing replicated pELUf1-BPDE. Cells transformed by the vector pELUf1 without the damaged oligonucleotide insert remained URA3 wild-type and thus could not grow on plates containing 5-FOA. After incubation at 30°C for 3–4 days, yeast colonies were counted. In each experiment with each strain, transformation efficiency was determined by a parallel transformation using the undamaged and double-stranded pELUf1. Translesion synthesis was calculated as transformants per µg of the damaged plasmid per 106 transformable cells with the undamaged plasmid (i.e. transformants per µg of the damaged plasmid x 106/transformation efficiency expressed as transformants per µg of the undamaged plasmid). Relative translesion synthesis was obtained by comparing translesion synthesis in various mutant strains to that in the wild-type cells.

Yeast colonies on the 5-FOA plates were individually resuspended in 10 µl of a solution containing 1 mg/ml yeast lytic enzyme in sterile water. After incubation at 37°C for 1.5–2 h, an aliquot of 1 µl was used for PCR amplification of a 670 bp plasmid region containing the original lesion site, using the primers, 5'-CCCGCAGAGTACTGCAATTTGAC and 5'-GAGCGGATAACAATTTCACACAGG. After heating the PCR mixture (20 µl) at 94°C for 4 min, 35 cycles of amplification were performed according to the following conditions: 30 s denaturation at 94°C, 30 s annealing at 65°C, and 45 s extension at 72°C. After the last cycle, the reaction was continued for 7 more min at 72°C. An aliquot of 2 µl PCR products was separated by electrophoresis on a 1% agarose gel containing 0.5 µg/ml ethidium bromide. Amplified DNA was purified by using the Wizard PCR Preps DNA Purification Resin according to the manufacturer's instruction. The precise specificity of translesion synthesis opposite the lesion was determined by sequencing the PCR DNA fragment. Yeast colonies that did not yield PCR products and plasmid clones that did not contain the inserted oligonucleotide sequence were excluded from the calculations. A few transformants by the empty vector pELUf1 escaped selection by the 5-FOA plates, probably due to mutations somewhere in the vector URA3 gene.


   RESULTS
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
DISCUSSION
 REFERENCES
 
The Pol{zeta} pathway is the major mechanism for translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in yeast cells
An in vivo genetic assay slightly modified from that of Zhao et al. (37) was used to examine translesion synthesis of a site-specific (+)- or (–)-trans-anti-BPDE-N2-dG adduct in yeast cells. A 33mer oligonucleotide containing the site-specific adduct was ligated into a single-stranded plasmid, which was subsequently converted into the double-stranded form by in vitro synthesis of the complementary strand using dUTP instead of dTTP. After transformation of the site-specifically damaged plasmid into cells, the complementary strand was degraded as a result of extensive DNA strand cleavage at sites of uracil by the sequential actions of a uracil-DNA glycosylase and an AP endonuclease, converting the plasmid DNA back into the single-stranded form (37). It is thus expected that this assay specifically reflects translesion synthesis without interference by DNA repair and template switching mechanisms (37), both of which require double-stranded DNA. The transformation efficiency was determined by using undamaged and double-stranded plasmids in the same experiment. After normalizing for transformation efficiency, the translesion synthesis efficiency in various cells relative to that in the wild-type cells was calculated. Yeast colonies were separately analyzed by PCR to recover a 670 bp region of the replicated plasmid. The specificity of translesion synthesis was determined by sequencing the PCR-amplified DNA fragments.

To examine the role of the Pol{zeta} pathway in bypassing the (+)- and (–)-trans-anti-BPDE-N2-dG adducts, we performed in vivo translesion synthesis assays in yeast rev3 (lacking Pol{zeta}) and rev1 deletion mutant cells. In rev1 mutant cells, the translesion synthesis of the (+)-trans-anti-BPDE-N2-dG adduct was reduced to 49% of the wild-type level (P = 0.005), while that of the (–)-trans-anti-BPDE-N2-dG adduct was reduced to 22% (Figure 1). In rev3 mutant cells, translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts were reduced to a greater extent between 16 and 6.5%, respectively, of the wild-type levels (Figure 1). In wild-type cells, translesion synthesis of the (–)-trans-anti-BPDE-N2-dG adduct occurred consistently at a slightly higher efficiency than that of the (+)-trans-anti-BPDE-N2-dG adduct by ~1.3-fold. These results show that the Pol{zeta} pathway is the major mechanism for translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in yeast cells.


Figure 1
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  		Figure 1 Relative frequencies of translesion synthesis (TLS) in various yeast strains. Using the plasmid pELUf1-BPDE containing a site-specific (+)- or (–)-trans-anti-N2-dG adduct, in vivo translesion synthesis assays were performed as described in Materials and Methods. Relative TLS was obtained by comparing translesion synthesis in various mutant strains to that in the wild-type cells. Slightly different transformation efficiencies as determined with the undamaged pELUf1 were taken into account in calculating the relative efficiencies. SDs are shown as error bars. WT, wild-type; rad30, lacking Pol{eta}; rev1, lacking Rev1; rev3, lacking Pol{zeta}; rev3rad30, lacking both Pol{zeta} and Pol{eta}.

 
Contribution of Pol{eta} to translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in vivo
To determine whether Pol{eta} affects bypass of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in yeast cells, we performed in vivo translesion synthesis assays in the rad30 deletion mutant strain (lacking Pol{eta}). As shown in Figure 1, in the absence of Pol{eta}, translesion synthesis of the (+)-trans-anti-BPDE-N2-dG adduct was reduced to 74% (±18%) of the wild-type level (P = 0.004), while that of the (–)-trans-anti-BPDE-N2-dG adduct was reduced to 63% (±6%). To gain insights into the genetic relationship between Pol{eta} and Pol{zeta} in bypass of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts, we examined translesion synthesis in rev3 rad30 double mutant cells that lacked both Pol{eta} and Pol{zeta}. As shown in Figure 1, translesion synthesis of either (+)- or (–)-trans-anti-BPDE-N2-dG in the double mutant cells was no more deficient than that in rev3 mutant strain. These results show that Pol{eta} plays a minor role in translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in yeast cells, and suggest that Pol{eta} functions in the Pol{zeta} pathway in response to these lesions.

Mutagenic translesion synthesis in yeast cells of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts predominantly results from A and G insertions opposite the lesions
To determine translesion synthesis products at a nucleotide resolution, we amplified by PCR a 670 bp region surrounding the original lesion site from individual yeast colonies, and subsequently sequenced the amplified DNA fragments. In wild-type cells, the majority of translesion synthesis resulted from the correct C insertion opposite the (+)- (76%) and (–)-trans-anti-BPDE-N2-dG (89%) adducts (Tables 1 and 2).While 10 and 7.3% of A and G, respectively, were inserted opposite (+)-trans-anti-BPDE-N2-dG (Table 1), only 4% each of A and G were inserted opposite (–)-trans-anti-BPDE-N2-dG (Table 2). Insertion of T, deletions, and tandem base substitutions constituted very minor translesion synthesis products (Tables 1 and 2). Other types of translesion synthesis, accounting for 3.6% and 0.9% for the (+)- and (–)-trans-anti-BPDE-N2-dG adducts, respectively (Tables 1 and 2), resulted from the correct C insertion opposite the lesion, but contained mutations at adjacent sites (Figure 2). For simplicity, these are referred to as untargeted mutagenesis products (Figure 2). These results indicate that mutagenic translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in yeast cells predominantly results from A and G insertions opposite the lesion, leading to G->T and G->C transversion mutations, respectively; and that the (+)-trans-anti-BPDE-N2-dG adduct is more mutagenic than the (–)-trans-anti-BPDE-N2-dG adduct.


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  		Table 1 Specificity of translesion synthesis opposite the (+)-trans-anti-BPDE-N2-dG adduct in various yeast strains

 

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  		Table 2 Specificity of translesion synthesis opposite the (–)-trans-anti-BPDE-N2-dG adduct in various yeast strains

 

Figure 2
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  		Figure 2 Products of untargeted mutagenesis. The damaged 33mer oligonucleotide contained in the plasmid pELUf1-BPDE is denoted as the template for translesion synthesis (TLS). Untargeted mutagenesis products resulting from TLS are shown below the damaged template. Strains from which the untargeted mutagenesis products were recovered are shown together with the number of such mutant plasmid clones. The correct C insertion opposite the lesion is indicated in blue, while mutations are illustrated in red. (A) Untargeted mutagenesis in the case of the (+)-trans-anti-N2-dG adduct. (B) Untargeted mutagenesis in the case of the (–)-trans-anti-N2-dG adduct. {Delta}, deletion; WT, wild-type; rad30, lacking Pol{eta}; rev3rad30, lacking both Pol{zeta} and Pol{eta}.

 
Contributions of Pol{zeta}, Rev1 and Pol{eta} to the mutagenic specificity of the (+)- and (–)-trans- anti-BPDE-N2-dG adducts
To gain insights into understanding the roles of Pol{zeta}, Rev1 and Pol{eta} in translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts, the bypass products were recovered by PCR from the corresponding yeast mutant strains, and the amplified DNA fragments were sequenced. Specificity of translesion synthesis in the absence of these bypass polymerases was then compared with that of the wild-type cells. Without Pol{eta}, insertion of A was largely reduced opposite the (+)-trans-anti-BPDE-N2-dG adduct (Table 1), and was not detected opposite the (–)-trans-anti-BPDE-N2-dG (Table 2). Additionally, insertion of G opposite the (+)-trans-anti-BPDE-N2-dG adduct was significantly increased (Table 1). Translesion synthesis in rev1 and rev3 (lacking Pol{zeta}) mutant cells yielded similar products. In rev3 mutant cells, insertion of G opposite the lesion was not detected, and insertion of A was significantly reduced (Tables 1 and 2). Similarly, in rev1 mutant cells, insertion of A opposite the lesion was significantly reduced, and insertion of G was not detected opposite the (+)-trans-anti-BPDE-N2-dG or greatly reduced opposite the (–)-trans-anti-BPDE-N2-dG adduct (Tables 1 and 2). In the absence of both Pol{eta} and Pol{zeta} (rev3 rad30 double mutant), insertions of A and G opposite the lesions were abolished (Tables 1 and 2).

When the altered bypass in the mutant strains was directly compared with the bypass of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in wild-type cells, expressed as translesion synthesis relative to that in wild-type cells, a major contribution by Pol{eta} to A insertion and the general dependence of mutagenesis on Rev1 and Pol{zeta} were clearly seen (Tables 3 and 4). The effect of Pol{eta} on G incorporation, however, differed for (+)- versus (–)-trans-anti-BPDE-N2-dG. In the absence of Pol{eta}, G insertions were increased by ~2-fold opposite the former lesion, but decreased ~4-fold opposite the latter lesion (Tables 3 and 4). Apparently, the untargeted mutagenesis (Figure 2) induced by (+)- and (–)-trans-anti-BPDE-N2-dG adducts, which constituted only a very minor fraction of the overall translesion synthesis, required Pol{zeta} and Rev1, but was independent of the Pol{eta} function. As for error-free translesion synthesis, reflected by C insertion opposite the lesion, Pol{eta} did not have a major impact. In contrast, such error-free bypass significantly depended on Rev1 and mostly required the function of Pol{zeta} (Tables 3 and 4).


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  		Table 3 Changes in translesion synthesis specificity opposite the (+)-trans-anti-BPDE-N2-dG adduct in mutant cells relative to that in wild-type cellsa

 

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  		Table 4 Changes in translesion synthesis specificity opposite the (–)-trans-anti-BPDE-N2-dG adduct in mutant cells relative to that in wild-type cellsa

 
Taken together, these results show that (i) Pol{eta}, Pol{zeta} and Rev1 combined are required for G->T mutagenesis induced by the (+)- and (–)-trans-anti-BPDE-N2-dG adducts; and (ii) G->C mutagenesis induced by these two lesions is generated through the Pol{zeta} pathway, independent of Pol{eta} opposite (+)-trans-anti-BPDE-N2-dG, but involving the Pol{eta} function opposite (–)-trans-anti-BPDE-N2-dG. Additionally, these results suggest that the Pol{zeta} pathway is the major mechanism, while Pol{eta} plays a minor role, for error-free translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts.


   DISCUSSION
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
REFERENCES
 
Pol{eta} was originally discovered as an error-free translesion synthesis polymerase in response to ultraviolet (UV)-induced TT dimers (39,40). However, the first clue suggesting that this polymerase may be involved in error-prone translesion synthesis of benzo[a]pyrene DNA adducts came from biochemical studies using templates containing a site-specific (+)-trans-anti-BPDE-N2-dG adduct (27). Purified Pol{eta} is able to recognize this bulky DNA lesion, and predominantly inserts an A opposite the lesion (27). Similar studies with the (–)-trans-anti-BPDE-N2-dG adduct showed that this lesion is less efficiently bypassed by Pol{eta} than the (+)-trans-anti-BPDE-N2-dG adduct (23,28,29). In this study, we tested our hypothesis that Pol{eta} participates in error-prone translesion synthesis by inserting A opposite the (+)- and (–)-trans-anti-BPDE-N2-dG adducts. Using an in vivo genetic assay that is based on the replication of site-specifically damaged plasmids in yeast cells, we showed that Pol{eta} indeed plays a major role in the insertion of A opposite these two stereoisomeric lesions, resulting in G->T transversion mutations. Therefore, in contrast to its anti-mutagenic role in response to UV radiation, Pol{eta} functions to promote mutagenesis induced by the (+)- and (–)-trans-anti-BPDE-N2-dG DNA adducts.

Insertions of A opposite the (+)- and (–)-trans-anti-BPDE-N2-dG adducts are mostly abolished when either Pol{eta}, Pol{zeta} or Rev1 is absent. Thus, the induced G->T transversion mutations depend on the functions of all these three proteins. Our in vivo results are best explained by the two-polymerase two-step mechanism of translesion synthesis (18,41). In such a bypass pathway, A is inserted by Pol{eta} opposite the lesion followed by extension synthesis catalyzed by Pol{zeta}. Supporting this conclusion, A is effectively inserted opposite (+)- and (–)-trans-anti-BPDE-N2-dG adducts by Pol{eta} in vitro (15,23,27–29), but not by Rev1, or just barely by Pol{zeta} (15,42). However, purified Pol{eta} is ineffective in extension synthesis from opposite these lesions (15,23,27,28). The bypass intermediate is thus most likely extended by Pol{zeta}. According to this mechanism, the insertion step would not occur without Pol{eta} and the extension step would not occur without Pol{zeta}, thus explaining the observed dependence on both Pol{eta} and Pol{zeta} of the G->T transversion mutations induced by the (+)- and (–)-trans-anti-BPDE-N2-dG adducts.

A very weak activity is detectable for Pol{eta} to extend from opposite the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in vitro (15,23,27,28). This marginal activity was most likely responsible for the residual (5–10% of the wild-type level) G->T transversion mutations in the absence of Pol{zeta} (Tables 3 and 4). In the absence of Pol{eta}, a residual A insertion activity was also observed opposite the (+)-trans-anti-BPDE-N2-dG adduct (Table 3), which probably resulted from the very weak activity of A insertion by Pol{zeta} (15). Supporting this interpretation, the residual A insertion activity was eliminated in cells lacking both Pol{eta} and Pol{zeta} (Tables 3 and 4).

With respect to G->T transversion mutagenesis induced by the (+)- and (–)-trans-anti-BPDE-N2-dG adducts, the phenotype of the rev1 mutant is similar to that of rev3 that lacks Pol{zeta}. Since Rev1 is unable to insert an A opposite these adducts (42), this protein must play a non-catalytic function for the induced G->T mutagenesis, which is similar to its role in UV-induced mutagenesis (42,43). So far, an in vivo catalytic activity (dCMP transferase) of Rev1 has been suggested only for the bypass of AP sites (37,43). Thus, the non-catalytic function of Rev1 may be responsible for its ubiquitous role in translesion synthesis in cells. The precise nature of this non-catalytic function, however, remains unknown.

In addition to A insertion, another major mode of mutagenic translesion synthesis is G insertion opposite the (+)- and (–)-trans-anti-BPDE-N2-dG adducts, leading to G->C transversion mutations, which requires the Pol{zeta} pathway. Missing either Pol{zeta} or Rev1 abolishes this mode of translesion synthesis. Opposite the (+)-trans-anti-BPDE-N2-dG adduct, Pol{zeta} is capable of translesion synthesis and preferentially inserts G in vitro (15), whereas purified Pol{eta} is inefficient in G insertion (15,23,27,28). Thus, we conclude that the observed G->C transversion mutations are generated mainly by Pol{zeta}-catalyzed G insertion followed by Pol{zeta}-catalyzed extension through the Pol{zeta} pathway, i.e. a one-polymerase two-step mechanism. Supporting this conclusion, inactivating Pol{eta} led to an increase in G->C mutagenesis, probably due to the elimination of the competitive binding of Pol{eta} to the template and Pol{eta}-catalyzed A insertion. In the case of the (–)-trans-anti-BPDE-N2-dG adduct, Pol{zeta} is also capable of translesion synthesis and preferentially inserts G in vitro, but its activity is lower than in the case of the stereoisomeric (+)-trans-anti-BPDE-N2-dG lesion (15). On the other hand, G insertion opposite (–)-trans-anti-BPDE-N2-dG catalyzed by yeast Pol{eta} is more active than opposite (+)-trans-anti-BPDE-N2-dG in vitro (15). Consistent with these biochemical results, we observed that G->C mutagenesis induced by (–)-trans-anti-BPDE-N2-dG was significantly reduced in yeast cells in the absence of Pol{eta} (Table 4), suggesting a major role for Pol{eta} in catalyzing G insertions opposite this lesion. Thus, the precise mechanism of G->C mutagenesis induced by the (+)-trans-anti-BPDE-N2-dG adduct is somewhat different from that induced by the (–)-trans-anti-BPDE-N2-dG adduct, due to the different catalytic selectivity of Pol{eta}.

Translesion synthesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in yeast cells results mainly in base incorporation opposite the lesions. Furthermore, most bypass products result from the correct C insertion opposite the lesion, i.e. error-free translesion synthesis. In vitro, Pol{eta}, Pol{zeta} and Rev1 all have significant C insertion activities opposite these two lesions (15,23,27,28,42). In this study, we found that C insertions opposite the lesions in vivo were only slightly reduced in the absence of Pol{eta}, but were more significantly reduced in the absence of Rev1, and mostly abolished in the absence of Pol{zeta} (Tables 3 and 4). Therefore, error-free translesion synthesis of these lesions is mostly performed by the Pol{zeta} pathway. The C insertion step is likely catalyzed by both Rev1 and Pol{zeta}. Since Rev1 cannot perform extension synthesis following C insertion (42), the extension step must be catalyzed by Pol{zeta}. Consistent with this interpretation, error-free bypass is most deficient in yeast cells lacking Pol{zeta}. Pol{eta} only makes a minor contribution (~30%) to error-free bypass of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts, for which the most likely mechanism is C insertion by Pol{eta} and subsequent extension by Pol{zeta}. A mechanistic model for translesion synthesis and mutagenesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts is summarized in Figure 3.


Figure 3
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  		Figure 3 Mechanistic models for translesion synthesis of the (+)- and (–)-trans-anti-N2-dG DNA adducts in yeast cells. The (+)-and (–)-trans-anti-BPDE-N2-dG adducts strongly block the replication complex (represented by the filled blue oval). Translesion synthesis is mediated predominantly by C insertion opposite the lesion catalyzed by the Rev1 dCMP transferase and by Pol{zeta}. Extension synthesis by Pol{zeta} completes the lesion bypass. Pol{eta} makes a minor contribution (~30%) to error-free bypass, for which the most likely mechanism is C insertion by Pol{eta} and subsequent extension by Pol{zeta}. Less frequently, A is inserted opposite the lesion by Pol{eta}, which is subsequently extended by Pol{zeta}, leading to G->T transversion mutations. Insertion of G opposite the lesions occurred at a low frequency, which is catalyzed by Pol{zeta} in the case of the (+)-trans-anti-BPDE-N2-dG adduct and by Pol{zeta} and Pol{eta} in the case of the (–)-trans-anti-BPDE-N2-dG adduct. Subsequent extension is catalyzed by Pol{zeta}, leading to G->C transversion mutations. Rev1 likely facilitates Pol{zeta}-catalyzed extension. In this role, Rev1 may play a non-catalytic function independent of its dCMP transferase.

 
The results with the site-specific (+)- and (–)-trans-anti-BPDE-N2-dG adducts in this study are in general agreement with our earlier report on mutagenesis induced by racemic (±)-anti-BPDE using randomly damaged plasmids (15). However, two differences are noted: quantitatively more G->C transversion mutations and deletions and insertions of 1–3 nt in the randomly damaged system (15). Two factors may be responsible for these differences. First, treatment of DNA with (±)-anti-BPDE yields multiple types of lesions. In addition to the (+)- and (–)-trans-anti-BPDE-N2-dG adducts, the (+)- and (–)-cis-anti-BPDE-N2-dG adducts and other minor BPDE adducts are also formed (2,4). These additional forms of DNA lesions likely contribute to the mutation spectrum. Secondly, deletions and insertions induced by (±)-anti-BPDE occur more frequently within single nucleotide repeats (15). The (+)- and (–)-trans-anti-BPDE-N2-dG adducts in this study, however, were not positioned within such repeat sequences. Consequently, it is expected that frameshift mutagenesis is less likely to occur in the sequence context examined in this study.

Our studies further suggest that the (+)-trans-anti-BPDE-N2-dG adduct is more mutagenic than the (–)-trans-anti-BPDE-N2-dG adduct. Since the two lesions did not differ greatly in translesion synthesis efficiency, the less mutagenic property of the (–)-trans-anti-BPDE-N2-dG adduct is likely a direct result of its more frequent error-free bypass in cells. A greater mutagenic potential of the (+)-trans-anti-BPDE-N2-dG adduct was also reported in cultured simian kidney cells in different sequence contexts (12). Hence, the mutagenecity of the BPDE adduct is influenced by its stereochemistry. Moreover, the specificity of nucleotide incorporation opposite the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in these mammalian cells was similar to that reported here in yeast cells. Recently, it was reported that (±)-anti-BPDE-induced mutagenesis in cultured human cells is largely abolished when Pol{zeta} and REV1 expressions are suppressed (32,33). These similarities suggest that the fundamental mechanism of translesion synthesis and mutagenesis of the (+)- and (–)-trans-anti-BPDE-N2-dG adducts is conserved from yeast to mammals. By comparing results using the same experimental system, it is possible for us to evaluate the intrinsic mutagenic potential of various DNA lesions. Thus, it is apparent that the p-benzoquinone DNA adducts derived from benzene are intrinsically much more potent in mutagenesis than the (+)- and (–)-trans-anti-BPDE-N2-dG adducts in yeast (44).


   ACKNOWLEDGEMENTS
 
We thank Zhongwen Xie for technical assistance. This work was supported by NIH grants CA92528 (to Z.W.), and CA20851 (to N.E.G.). Funding to pay the Open Access publication charges for this article was provided by the NIH grant CA92528.

Conflict of interest statement. None declared.


   REFERENCES
TOP
 ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
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