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Corrigendum for Yun et al., Nucl. Acids Res. 34 (12) e85.
Nucleic Acids Research 2006 34(22):6718; doi:10.1093/nar/gkl1020
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Nucleic Acids Research, 2006, Vol. 34, No. 22 6718
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corrigendum

Corrigendum

Nucleic Acids Res. (2006) 34, e85

Genomic DNA functions as a universal external standard in quantitative real-time PCR

J. J. Yun, L. E. Heisler, I. I. Hwang, O. Wilkins, S. K. Lau, M. Hyrcza, B. Jayabalasingham, J. Jin, J. McLaurin, M. S. Tsao and S. D. Der

The authors apologize for an error in Materials and Methods in the above paper.

The final concentration of primers in a single real-time PCR should have been written as 0.5 µM (micro) not 1 nM (nano).

The full and correct sentence is given below.

Each 10 ml reaction contained 1x PCR buffer (Sigma–Aldrich Co.), 3 mM MgCl2, 0.2 mM dNTP, 0.5 µM forward and reverse primers, 1:50 dilution of ROX reference dye (Sigma–Aldrich Co.), 3:100 000 dilution of SYBR Green I (Sigma–Aldrich Co.), 0.05 U of JumpStart Taq polymerase (Sigma–Aldrich Co.) and template DNA.


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This Article
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