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Corrigendum for Demeshkina et al., Nucl. Acids Res. 35 (14) 4597-4607.

Nucleic Acids Research Advance Access originally published online on October 11, 2007
Nucleic Acids Research 2007 35(20):7040; doi:10.1093/nar/gkm716
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Nucleic Acids Research, 2007, Vol. 35, No. 20 7040
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corrigendum

Novel activity of eukaryotic translocase, eEF2: dissociation of the 80S ribosome into subunits with ATP but not with GTP

Natalia Demeshkina, Go Hirokawa, Akira Kaji and Hideko Kaji

Nucleic Acids Res. (2007) 35, 4597–4607.

The authors wish to apologize for an error in Figure 4C. The time-scale on the X axis should be ‘min’ instead of ‘sec’. A corrected version of Figure 4 and its figure legend are given below.


Figure 1
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  		Figure 4. ATP but not other nucleotides are essential for the eEF2-dependent dissociation of 80S ribosomes and the binding of eEF2 to subunits: ATP hydrolysis during the splitting. (A) Ribosomes (0.05 µM) were pre-incubated in buffer 2/150 with 2.5 µM eEF2 and 0.5 mM of one of the specified nucleotides for 6 min at 30°C and then eIF6 was added to 2 µM and further incubated for 15 min. The reaction mixtures were analyzed by SDGC. The percentages of dissociation of 80S ribosomes were determined as in Figure 3A. Standard error was ±5%. (B) Reaction mixtures (15 µl) containing 0.05 µM 80S ribosomes and 2.5 µM eEF2 were incubated at 30°C with 0.5 mM [{gamma}-32P]ATP in buffer as indicated. Sordarin was purchased from Sigma. (C) Ribosomes were incubated with eEF2 as in (B) with indicated amounts of [{gamma}-32P]ATP in buffer 2/150. (D) Ribosomes (0.05 µM) were incubated with eEF2 (1 µM) and 0.5 mM nucleotide as indicated in buffer 2/150 followed by treatment with 0.45% glutaraldehyde (v/v) and SDGC. The presence of eEF2 in each fraction was estimated by western blotting. In (B) and (C), each curve represents the average of three independent experiments.

 

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This Article
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Right arrow Screen PDF (140K) Freely available
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gkm716v1
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