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Figure 2. Stretching of surface-tethered DNA using continuous flow. (A) Schematic of the assay. DNA is attached to the glass surface with one end. To overcome the entropic forces that keep the DNA compact a continuous solvent flow is applied, extending the DNA. Visualization of DNA or associated proteins can be realized using fluorescence microscopy. (B) Application of this assay to YOYO-stained 
-DNA (21), demonstrating how DNA stretching and extension depends on the flow rates. It furthermore highlights ability of this approach to allow the simultaneous observation of many DNA molecules in one field of view. Adapted with permission from Ref. (21). Copyright 2006 American Chemical Society.
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