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Nucleic Acids Research, 1982, Vol. 10, No. 1 1-17
© 1982

Articles

RSITE: a computer program to predict the recognition sequence of a restriction enzyme

Carolyn M. Tolstoshev and Robert W. Blakesley

Bethesda Research Laboratories, Inc. P.O.Box 577, Gaithersburg, MD 20877, USA

Received July 24, 1981. A computer program (RSITE) was developed which predicts the recognition sequence of a restriction endonuclease. The sizes of fragments experimentally determined on cleavage of a DNA of known sequence were input. Possible recognition sequences producing fragments of sizes matching those determined empirically were printed Out. The program faithfully predicted the specificity of restriction enzymes of known recognition sequence and also determined the recognition sequence of a new restriction enzyme from Haemophilus influenzae GU (HinGU II).


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