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Nucleic Acids Research, 1982, Vol. 10, No. 11 3475-3486
© 1982

MOLECULAR BIOLOGY

Effect of in vitro methylation at CpG sites on gene expression in a genome functioning autonomously in a vertebrate host

Kiranur N. Subramanian

Department of Microbiology and Immunology, University of Illinois at the Medical Center Chicago IL 60612, USA

Received December 11, 1981. Revised May 4, 1982. Accepted May 4, 1982.

The effect of in vitro methylation at the Hpa II sites in polyoma DNA on viral gene expression and the maintenance of the methyl groups upon replication in vivo were examined. Most of the methylatable sites are located in the early region coding for the viral large T antigen which is essential for the replication and infectivity of the viral DNA, Methylated or mock-methylated polyoma DNA produced the same nunber of virus plaques appearing at the same time post-transfection in either case. The lack of effect on the infectivity of the viral DNA indicates that the expression of the T antigen gene was not inhibited by methylation. Replication in vivo of the DNA also resulted in a total loss of the methyl groups introduced in vitro These results underscore basic differences between the behavior of an autonanously functioning papovavirus DNA and the animal cell DNA vis-a-vis methylation at CpG sites. These differences might be due to subtle variations in the mechanian of regulation of gene expression and replication in the two systems.


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K. Hoelzer, L. A. Shackelton, and C. R. Parrish
Presence and role of cytosine methylation in DNA viruses of animals
Nucleic Acids Res., May 1, 2008; 36(9): 2825 - 2837.
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