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Nucleic Acids Research, 1982, Vol. 10, No. 14 4247-4257
© 1982

MOLECULAR BIOLOGY

Restriction fragment length polymorphism of the rat albumin gene in Sprague-Dawley rats and its application in genetic study of analbuminemia

Hiroyasu Esumi1, Yuri Takahashi2, Shigeaki Sato2 and Takashi Sugimura2

1Virology Division, National Cancer Center Research Institute 5-1-1, Tsukiji, Chuo-ku, Tokyo, Japan 2Biochemistry Division, National Cancer Center Research Institute 5-1-1, Tsukiji, Chuo-ku, Tokyo, Japan

Received April 20, 1982. Revised June 14, 1982. Accepted June 14, 1982.

Restriction fragment length polymorphism of the rat albumin gene was discovered in a stock of Sprague-Dawley rats by Southern blots of rat liver DNAs using cloned albumin cDNA, prAlb-1 (1), as a probe. The polymorphic DNA fragments were observed when rat DNAs were digested with eit her Hind III or Pst 1 and the difference in length of the DNA fragments in Hind III or Pst 1 digests was estimated as 1.4 kbp. When DNAs were digested with EcoR I, restriction fragment length polymorphism was not observed. Therefore, this polymorphic DNA was concluded to be located in the flanking sequence. Structural analysis of the cloned albumin gene showed that the polymorphism was located in the 3'-flanking sequence. With this polymorphism as a marker of the albumin structural gene, the phenotype of analbuminemia, which is an autosomally recessive trait , was found to be linked to the structural gene of albumin.


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