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Nucleic Acids Research, 1983, Vol. 11, No. 10 3333-3346
© 1983

CHEMISTRY

Assignment of non-exchangeable base proton and H1‘ resonances of a deoxyoctanucleoside heptaphosphate d(G-G-G*-G*G-G-C-C-) by using the nuclear Overhauser effect

M.R. Sanderson, J.R. Mellema, G.A. Van der Marel, G. Whille, J.H. Van Boom and C. Altona

Gorlaeus Laboratories, State University of Leiden P.O. Box 9502, 2300 Leiden, The Netherlands

Received April 11, 1983. Accepted April 25, 1983.

The resonances of the non-exchangeable base protons and I1protons of the octamer d(G-G-C*-C*-G-G-C-C), C*m5dC, have been assigned by means of NOE difference NMR spectroscopy at 500 MHz. From the measured Jl'21 and J1'2" it follows that the octamer at low temperature prefers to adopt a B-DNA double-helical conformation in solution, however, some residual conformational freedom is detected at the 3'terminus. From the chemical shift versus temperature profiles it is concluded that no major conformational change occurs below 60-65 °C where the duplex formation for residues (2) to (6) is essentially completed under the conditions used.


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