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Nucleic Acids Research, 1983, Vol. 11, No. 11 3651-3663
© 1983

MOLECULAR BIOLOGY

Organization of gene and non-gene sequences in micronuclear DNA of Oxytncha nova

Robert E. Boswell*, Carolyn L. Jahn, Arthur F. Greslin and David M. Prescott

Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, CO 80309, USA

Received March 7, 1983. Revised May 2, 1983. Accepted May 2, 1983.

In order to study the derivation of the macronuclear genome from the micronuclear genome in Oxytricha nova micronuclear DNA was partially digested with EcoRI, size fractionated, and then cloned in the lambda phage Charon 8. Clones were selected a) at random b) by hybridization with macro-nuclear DNA or c) by hybridization with clones of macronuclear DNA. One group of these clones contains only unique sequence DNA, and all of these had sequences that were homologous to macronuclear sequences. The number of macronuclear genes with sequences homologous to these micronuclear clones indicates that macronuclear sequences are clustered in the micronuclear genome. Many micronuclear clones contain repetitive DNA sequences and hybridize to numerous EcoRI fragments of total micronuclear DNA, yielding similar but non-identical patterns. Some micronuclear clones containing these repetitive sequences also contained unique sequence DNA that hybridized to a macronuclear sequence. These clones define a major interspersed repetitive sequence family in the micronuclear genome that is eliminated during formation of the macronuclear genome.

*Present address: National Institute of Environmenetal Health Services, Labortarory of Animal Genetics P.O. Box 12233, Research Triangle Park, NC 27709. USA


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