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Nucleic Acids Research, 1983, Vol. 11, No. 11 3687-3702
© 1983

ENZYMOLOGY

Properties of a maonovalent and divalent requiring endoribonuclease with novel specificity from calf heart

Eiko Akaboshi1 and Sidney Altman

Depanment of Biology, Yale University New Haven, CT 06520, USA

Received February 15, 1983. Revised April 20, 1983. Accepted May 6, 1983.

A new sonovalent and divalent requiring endoribonuclease hat been identified in extracts of calf heart titans using a natural substrate nolecule. This ribonuclease creates 5– hydroxyl and 3– phosphate groups in its cleavage products. It cleaves predominantly between pyrimidine and A residues though not all such possible sites are attacked in the substrates tested.

1Present address: Laboratory of Molecular Genetics, School of Medicine, Osaka University, Osaka, Japan


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